The induced fit and conformational selection/population shift models are two extreme cases of a continuum aimed at understanding the mechanism by which the final key-lock or active enzyme conformation is achieved upon formation of the correctly ligated enzyme. Structures of complexes representing the Michaelis and enolate intermediate complexes of the reaction catalyzed by phosphoenolpyruvate c...

Phosphoenolpyruvate carboxykinase (EC 4.1.1.32) has been isolated and purified from the cytosol fraction of rat liver to give a specific activity of 14 to 16 moles of bicarbonate tied per min per mg of protein. The enzyme is homogeneous on disc electrophoresis and Sephadex G-100 chromatography and has a molecular weight of 74,500 with an isoelectric point of 5.04. Antibodies prepared in rabbits...

We used a nuclear RNA transcript elongation assay to show that cAMP analogs and dexamethasone cause a selective increase of transcription of the P-enolpyruvate carboxykinase gene in H4IIE hepatoma cells. 8-(4-~hlorophenylthio)-cAMP increased transcription within 5 min and the maximal rate, generally 10-15fold above the basal rate, was attained by 30 min. This increase was of sufficient magnitud...

Much has been published over the years on the enzymology, genetics, and metabolic role of the enzyme phosphoenolpyruvate carboxykinase (GTP) (PEPCK; EC 4.1.1.32), and much of it has been in the Journal of Biological Chemistry. It is thus not an accident that the Journal is again focusing on this important enzyme in a Compendium that includes the original article by Utter and Kurahashi describin...