Phospholipids are key molecules that contribute to the structural definition of cells and that participate in the regulation of cellular processes. Phospholipid metabolism is a major activity that cells engage in throughout their growth. The yeast, Saccharomyces cerevisiae, serves as a model system in which to study the regulation of phospholipid synthesis and its regulation in eucaryotes. Its ...

Although it is well known that phospholipids self-assemble on hydrophilic plasma-oxidized PMDS surfaces (ox-PDMS) to form cell membrane mimetic bilayers, the temporal stability of phospholipid membranes on these surfaces is unknown. Here we report that phospholipid bilayers remain stable on solvent-cleaned ox-PDMS for at least 132 hours after preparation. Absent solvent cleaning, the bilayers w...

The role of phospholipid in rat liver microsomal glucose 6phosphatase has been investigated with the use of phospholipase A and phospholipase C to alter the microsomal phospholipids. The phospholipase C-treated preparation lost a maximum of 80 to 90% of the original activity, and 70% of the microsomal phospholipid was hydrolyzed. Addition of phospholipid completely restored the original activit...

Many attempts have been made to influence the phospholipid content of various organs of the body by the administration of phospholipid. Reports of results obtained upon feeding phospholipids are many and conflicting, one reason being, as pointed out by Sinclair (1934), that it is highly improbable that any intact phospholipid would be absorbed from the intestinal tract. The intravenous injectio...

A simple, rapid micro-method, suitable for use in a routine clinical laboratory, is described for isolating a surfactant fraction from 0.1 mL of human amniotic fluid and measuring its phospholipid content. We determined the phospholipid content of this fraction, referred to as "lamellar body phospholipid," in 451 samples of amniotic fluid collected within two days of delivery and related the da...

We studied the combined effect of sesamin (1:1 mixture of sesamin and episesamine) and soybean phospholipid on lipid metabolism in rats. Male rats were fed diets supplemented with 0 or 2 g/kg sesamin, and containing 0 or 50 g/kg soybean phospholipid, for 19 days. Sesamin and soybean phospholipid decreased serum triacylglycerol concentrations and the combination of these compounds further decrea...