BACKGROUND AND OBJECTIVE Artocarpus heterophyllus (jackfruit) is a latex producing plant. Plant latex is produced from secretory cells and contains many intergradients. It also has been used in folk medicine. This study aimed to purify and characterize the biological activities of a protease from jackfruit latex. MATERIALS AND METHODS A protease was isolated and purified from crude latex of a...

The barley nucellin gene was reported to be nucellus specific in its expression and was hypothesized to play a role in the programmed cell death of the nucellus as an aspartic protease. Here we provide direct evidence that the rice ortholog encodes an active aspartic protease, but we prefer the name aspartic protease1 (OsAsp1) to nucellin after a detailed analysis of its expression pattern in r...

Proteases regulate a large number of biological processes in plants, such as metabolism, physiology, growth, and defense. In this study, we carried out virus-induced gene silencing assays with pepper cDNA clones to elucidate the biological roles of protease superfamilies. A total of 153 representative protease genes from pepper cDNA were selected and cloned into a Tobacco rattle virus-ligation ...

Organic N mineralization by extracellular proteases affects inorganic N availability and loss. Soil Nmineralization is slowed by addition of purified protease inhibitors. We hypothesized that elevated concentrations of protease inhibitors in plant residues would reduce soil and plant residue N mineralization. Isogenic controls and transgenic plants of Brassica (Brassica napus L.), Japonicum ric...

Raphides, needle-shaped calcium oxalate crystals in tissues of many plants, have been thought to play defensive roles against herbivores without detailed bioassays for their defensive roles and modes of function using purified raphides. In order to examine the defensive roles and modes of function of raphides in a clear experimental system, we performed bioassays giving the larvae of the Eri si...

the antagonistic effects of rhizobium spp. isolates against rhizoctonia solani (ag-4), the causal agent of bean damping-off were compared with each other in either of the in vitro and in vivo conditions. also their potential to produce such secondary antifungal metabolites as hcn, siderophore and protease were evaluated. the results indicated that, isolate rh3 reduced mycelial growth of r. sola...

The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity...

This study is the first to demonstrate the activity of putative cellulosomal protease/peptidase inhibitors (named cyspins) of Clostridium cellulovorans, using the Saccharomyces cerevisiae display system. Cyspins exhibited inhibitory activities against several representative plant proteases. This suggests that these inhibitors protect their microbe and cellulosome from external attack by plant p...

Proteases, one among the three largest groups of industrial enzymes, accounts for about 60% of the total worldwide sale of enzymes from biological sources since they possess almost all characteristics desired for their biotechnological applications (Adinarayana et al., 2003). Among the various proteases, bacterial protease was the most significant compared with animal, fungi and plant protease....

the aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of argyrosomus regius larvae using in vitro techniques. a. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (dah)). the differences observed in protease activities of meagre larvae during the samplin...