Background: Urinary tract Infections caused by multidrug resistant uropathogens have become a significant global public health problem with Nigeria being no exception.
 Objective: This study is aimed at profiling and curing the plasmids of selected isolated from patients urinary infection in tertiary hospital Calabar, Nigeria.
 Methodology: Isolates were obtained urine samples using s...

Transcription initiation of the copy-number control and better-than-random segregation genes of the broad-host-range and low-copy-number plasmid pSM19035 are subjected to repression by the autoregulated pSM19035-encoded omega product in Bacillus subtilis cells. The promoters of the copS (Pcop1 and Pcop2), delta (Pdelta), and omega (Pomega) genes have been mapped. These promoters are embedded in...

Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...

Abstract Inappropriate use of antibiotics is globally creating public health hazards associated with antibiotic resistance. Bacteria often acquire resistance by altering their genes through mutation or acquisition plasmid-encoding genes. To treat drug-resistant strains bacteria, the recently developed CRISPR-Cas9 system might be an alternative molecular tool to conventional antibiotics. It disa...

The study aimed to investigate the occurrence of carbapenem resistant E. coli and P. aeruginosa in apparently healthy, captive blackbucks leopards India. Faecal samples (n = 7) were processed isolate (CRE) (CRP). Forty (leopards n 26; blackbuck 14) two (blackbuck 2) isolated from faecal 14). Eleven isolates recovered, which 10 CRE one was CRP. minimum inhibitory concentration (MIC) determined f...

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...

background: protozoa related to trypanosome family including leishmania , synthesize enzymes to escape from drug therapy. one of them is ptr1 that its enzymatic activity is similar to dihydrofolate reductase (dhfr). dihydrofolate reductase - thymidylate synthase has a major role in dna synthesis, if it is inhibited, the result would be the death of parasite. since ptr1 activity is similar to dh...

recent technological improvements have extended the application range of dielectric permittivity biomass measurements to on-line monitoring of physiological changes during bacterial fermentations. in an industrial fermentation of bacillus thuringiensis, it is important to verify the intactness of bacterial plasmid content in all steps of culture. changes in certain plasmid resident properties o...

in this essay, the heavy metal resistance patterns of bacterial strains isolated from industrial wastewater, domestic wastewater and various parts of the wastewater treatment system of arak city have been studied. 28 intestinal bacterial strains were screened and identified as klebsiella, escherichia coli, citrobacter and enterobacter species biochemical methods. the minimum inhibitory concentr...