The adsorption and assembly of B18 peptide on various solid surfaces were studied by reflectometry techniques and atomic force microscopy. B18 is the minimal membrane binding and fusogenic motif of the sea urchin protein bindin, which mediates the fertilization process. Silicon substrates were modified to obtain hydrophilic charged surfaces (oxide layer and polyelectrolyte multilayers) and hydr...

Some general aspects of the interaction of foreign materials with blood are summarized. The role of protein adsorption in this interaction is briefly discussed. In an attempt to produce more stable antithrombogenic surface coatings than the well-known heparin-bonded surfaces, a method is described in which a high-molecular-weight polyelectrolyte is bound to polymer surfaces. In vitro experiment...

Photothermal desorption of molecules from plasmonic nanoparticles is an example of a light-triggered molecular release due to heating of the system. However, this phenomenon ought to work only if the molecule-nanoparticle interaction is exothermic in nature. In this study, we compare protein adsorption behavior onto gold nanoparticles for both endothermic and exothermic complexation reactions, ...

Techniques are described for the coating of sorbents to be used in an artificial liver support system based on mixed sorbent bed hemoperfusion. Activated charcoal has been coated with cellulose acetate (CA) by solvent evaporation. With Amberlite XAD-4, the Wurster technique was used for coating with CA. XAD-4 has also been coated with a synthetic polyelectrolyte with anticoagulant activity by a...

Acid-resistant, nonenveloped viruses belonging to the enterovirus, reovirus, and adenovirus groups were readily concentrated on PE60, an insoluble cross-linked polyelectrolyte based on isobutylene maleic anhydride. Hydrolysis of PE60 by NaOH increased its capacity to adsorb viruses. Hydrogen ion levels played an important role in virus concentration; optimal pH levels for maximal virus adsorpti...

Polyelectrolyte capsules with silver and gold nanoparticles in the shell composition have been obtained using calcium carbonate and polystyrene microparticles as cores. Capsules were modified with silver nanoparticles using silver mirror reaction. Gold nanoparticles were embedded in capsule shells via their adsorption from previously obtained sol. A significant difference in the structure of ca...

−A new method is introduced to build up organic/organic multilayer films composed of cationic poly(allylamine hydrochloride) (PAH) and negatively charged poly(sodium 4-styrenesulfonate) (PSS) by using the spinning process. The adsorption process is governed by both the viscous force induced by fast solvent elimination and the electrostatic interaction between oppositely charged species. On the ...

Cell behavior in the presence of nanomaterials is typically explored through simple viability assays, but there is mounting evidence that nanomaterials can have more subtle effects on a variety of cellular functions. Previously our lab demonstrated that gold nanorods functionalized with polyelectrolyte multi-layers inhibited rat cardiac fibroblast-mediated remodeling of type I collagen scaffold...

Self-assembled monolayers of thiols on gold are employed to study the role of surface chemistry on adsorption of polyelectrolytes to solid substrates. The suitability of these substrates is demonstrated in optical reflectivity, which combines high sensitivity to the possibility to precisely control the hydrodynamic conditions at the solid/water interface. Therefore, this system allows the deter...

giant unilamellar vesicles (guvs) consisting in self-closed lipid bilayers of 0.5-100 µm diameter are considered as oversimplified models of cells because of their biological membrane and micrometric size while large unilamellar vesicles (luvs) of 100-500 nm diameter have applications in drug delivery. to improve structural and mechanical properties of these vesicles, we have developed two cate...