Fourteen polymorphic microsatellite loci were isolated in this transcriptome-based data analysis for Cotesia plutellae, which is an important larval parasitoid of the worldwide pest Plutella xylostella. A subsequent test was performed for a wild C. plutellae population (N = 32) from Fuzhou, Fujian, southeastern China, to verify the effectiveness of the 14 microsatellite loci in future studies o...

PREMISE OF THE STUDY Dioscorea alata L. is one of the most widely distributed species of the genus in the humid and semihumid tropics and is associated with traditional agriculture. Only a few microsatellite markers have been developed so far for this and other Dioscorea species. METHODS AND RESULTS We isolated 14 codominant polymorphic microsatellite markers using a microsatellite-enriched g...

The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% p...

Cowpea improvement has continued to play key roles in the quest for national food security. This work elucidated molecular genetic diversity and phylogenetics among four released cowpea varieties (FUAMPEA-1, FUAMPEA-2, FUAMPEA-3 AND FUAMPEA-4). DNA was extracted from 14 day old seedlings using CTAB method. A total of nine (9) SSR (Simple Sequence Repeats) markers were employed amplifications. B...

It is important to analyse the degree of genetic variation existing within genome extend base linseed/flaxseed accessions in order preserve, evaluate and use resources accurately successfully. The main aim current investigation was scope spread genomic across different linseed by employing molecular markers (RAPD). DNA 12 amplified with 16 decamer RAPD primers that generated 81 total bands, amo...

Pistachio is one of the most important horticultural products of Iran. The best way to get the maximum yield is to have genetically pure and monotonous gardens. Thereby, study of genetic variation and providing genetic identifications, make this possible to have homogenous gardens with high performance genotypes. In the present study, genetic diversity of 19 Iranian pistachio cultivars was asse...

Analysis of genetic diversity among genotypes and differentiation populations are crucial for determination conservation strategies one the best plant breeding approaches. In this study, ISSR markers were used to determine variation population structure in 23 hulled wheats. Together with control durum wheat bread registered cultivars, 32 analyzed 14 located throughout genome. Number alleles per...

Based on target traits, use of the genetic diversity rice is beneficial for crop improvement. In this study, 41 varieties local to Vietnam’s Mekong Delta were evaluated basis 11 quantitative morphological along with assessment according 50 SSR markers. The actual yield had a significance level 0.05, while plant height and panicles per square meter high 0.001. Cluster analysis based traits also ...

Aims: The aim of the study was to determine genetic diversity twenty four Boro rice landraces using genome specific twelve well known SSR markers.
 Study Design: Genomic DNA extraction, PCR amplification, Polyacrylamide gel electrophoresis (PAGE) and data analysis-these steps were followed perform research work. Data analysed with help following software; POWERMAKER version 3.25, AlphaEase...

In the present investigation 28 genotypes of soybean were analysed using 37 start codon targeted (SCoT) markers and primers produced 260 DNA fragments with an average 7.03 bands per primer. From these primers, SCoT 33 65 wos most polymorphic, where 10 polymorphic amplification products detected. The lowest number amplified (2) was detected by 8, 14, 19, 31 59. Of bands, 200 (77.27 %) 5.41 To de...