BACKGROUND Ischemic preconditioning reduces local tissue injury caused by subsequent ischemia-reperfusion (IR), but may also have a salutary effect on IR injury of tissues remote from those undergoing preconditioning. We tested the hypothesis that limb ischemia induces remote preconditioning, reduces endothelial IR injury in humans, and reduces experimental myocardial infarct size. METHODS AN...

Zhang, Hong Yan, Bradley C McPherson, Huiping Liu, Timir S Baman, Peter Rock, and Zhenhai Yao. H2O2 opens mitochondrial KATP channels and inhibits GABA receptors via protein kinase Cin cardiomyocytes. Am J Physiol Heart Circ Physiol 282: H1395–H1403, 2002; 10.1152/ajpheart.00683.2001.—Oxygen radicals and protein kinase C (PKC) mediate ischemic preconditioning. Using a cultured chick embryonic c...

Gastric ischemic preconditioning is one of the most effective approaches to attenuate the gastric mucosal injury caused by severe ischemia-reperfusion. In the present study we investigated whether glucocorticoid hormones participate in a realization of gastroprotective effect of ischemic preconditioning. Rats were exposed to prolonged gastric ischemia-reperfusion (30 min occlusion of celiac art...

OBJECTIVE To evaluate the effects of pharmacological preconditioning with a volatile anesthetic in patients undergoing liver resection with inflow occlusion. BACKGROUND In liver surgery, ischemic preconditioning and intermittent clamping are the only established protective strategies to reduce tissue damage due to ischemia during inflow occlusion. Preconditioning with volatile anesthetics has...

Transient exposure of rat cortical cultures to nonlethal oxygen-glucose deprivation (OGD preconditioning) induces tolerance to otherwise lethal oxygen-glucose deprivation (OGD) or N-methyl-D-aspartate 24 h later. This study evaluates the role of cytosolic and mitochondrial Ca2+-dependent cellular signaling. Mechanistic findings are placed in context with other models of ischemic preconditioning...

Background: Preconditioning is a powerful endogenous mechanism to protect the heart against ischemic damage. The second window of preconditioning (SWOP) is therapeutically the most attractive, but is hard to achieve by local cardiac ischemia 24 hrs before the index ischemia in the many mice models for cardiovascular pathology, because of excessive mortality during the two procedures or the peri...

BACKGROUND We have recently demonstrated that remote ischemic preconditioning reduces ischemia-reperfusion injury in animal models. The mechanisms by which the remote ischemic preconditioning stimulus exerts its effect remain to be fully defined, and its effect on myocardial gene expression is unknown. We tested the hypothesis that remote ischemic preconditioning modifies myocardial gene expres...

Hyperlipidemia attenuates the cardioprotective effect of preconditioning via unknown mechanisms. We have previously reported that in normolipidemic rats preconditioning decreased ischemia-induced activation and release of myocardial matrix metalloproteinase-2 (MMP-2) into the coronary perfusate. Here we investigated whether hyperlipidemia interferes with the cardioprotective effect of precondit...

Preconditioning is an experimental strategy for reducing ischemic brain damage. There are reports that brief exposure of neurons to NMDA-receptor antagonists may be an adequate preconditioning stressor. We studied effects of preconditioning of the cerebellar granule cells (CGC) in primary culture by 30-minute exposure to NMDA receptor antagonists 0.5 μM MK-801 or 5 μM memantine. CGC were challe...

OBJECTIVE Beta cells of pancreatic islets are susceptible to functional deficits and damage by hypoxia. Here we aimed to characterize such effects and to test for and pharmacological means to alleviate a negative impact of hypoxia. METHODS AND DESIGN Rat and human pancreatic islets were subjected to 5.5 h of hypoxia after which functional and viability parameters were measured subsequent to t...