Factors influencing basophil production from the bone marrow of ovalbumin (OA)-sensitized guinea pigs have been examined in vitro. Autologous co-cultures of marrow and spleen cells from OA-immune animals contained significantly higher numbers of basophils after 7 d of liquid culture in the presence of OA, compared with control co-cultures or with marrow cultures alone (P < 0.005). Basophils inc...

The assessment of nutritional intakes during hospitalization is crucial, as it is known that nutritional status tends to worsen during the hospital stay, and this can lead to the negative consequences of malnutrition. International guidelines recommend the use of parenteral nutrition (PN) in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition. Howe...

Microorganism accumulating polyhydroxyalkanoates (PHA) in its cytosol was isolated from water obtained from InCheon area. This strain was determined to be Bacillus species that was examined by 16S rDNA sequences and phylogenetic tree analysis. The bacteria accumulated up to 20.6% of PHA within 48 hours at 37 o C in the presence of 2.0% glucose substrate under aerobic condition. The present work...

Probabilistic Hazard Assessment (PHA) provides an appropriate methodology for assessing space weather hazards and their impact on technology. PHA is widely used in geosciences to determine the probability of exceedance critical thresholds, caused by one or more hazard sources. has proved useful with limited historical data estimate likelihood specific impacts. also driven development empirical ...

PHA-1 encodes a cytoplasmic protein that is required for embryonic morphogenesis and attachment of the foregut (pharynx) to the mouth (buccal capsule). Previous reports have in some cases suggested that PHA-1 is essential for the differentiation of most or all pharyngeal cell types. By performing mosaic analysis with a recently acquired pha-1 null mutation (tm3671), we found that PHA-1 is not r...

This work aimed to isolate a series of bacterial strains associated with the green seaweed Ulva sp. and evaluate their capability manufacture PHA. The effect type supplemented sugars found be in macroalgae, on growth PHA productivity was studied. Analysis 16S rRNA gene sequence isolated revealed that PHA-producing bacteria were phylogenetically related genus Cobetia, Bacillus, Pseudoaltermonas ...

BACKGROUND Fluorescence activated cell sorting (FACS) is a powerful technique for the qualitative and quantitative detection of biomolecules used widely in both basic research and clinical diagnostic applications. Beads displaying a specific antigen are used to bind antibodies which are then fluorescently labelled using secondary antibodies. As the individual suspension bead passes through the ...

The regulation of medium-chain-length polyhydroxyalkanoates (mcl-PHA) metabolism in Pseudomonas putida GPo1 was studied by analysis of enzymes bound to PHA granules and enzymes involved in fatty acid oxidation. N-terminal sequencing of granule-bound enzymes revealed the presence of PHA polymerase (PhaC) and PHA depolymerase (PhaZ) and an acyl-CoA synthetase (ACS1), which recently was found to b...

The photosynthetic cyanobacterium, Synechocystis sp. strain 6803, is a potential platform for the production of various chemicals and biofuels. In this study, direct photosynthetic production of a biopolymer, polyhydroxyalkanoate (PHA), in genetically engineered Synechocystis sp. achieved as high as 14 wt%. This is the highest production reported in Synechocystis sp. under photoautotrophic cult...