The purpose of these studies was to select and isolate cells with increased liver-metastasizing potential from heterogeneous primary human colon carcinomas (HCCs). Cells derived from a primary HCC classified as Dukes' stage B2 were directly established in culture or were injected into the subcutis, cecum, or spleen of nude mice. Progressively growing tumors were excised, dissociated, and establ...

Cholangiopathies are chronic liver diseases in which damaged cholangiocytes trigger a proinflammatory and profibrotic reaction. The nuclear vitamin D receptor (VDR) is highly expressed exerts immune-regulatory functions these cells. In the present study, we examined protective function of VDR other signaling pathways cholangiopathy cholangiocytes. Vdr was invalidated Abcb4 knockout mice, widely...

Objective(s): Liver transplantation is the most important therapy for end-stage liver disease and ischemia reperfusion (I/R) injury is indeed a risk factor for hepatic failure after grafting. The role of miRNAs in I/R is not completely understood. The aim of this study was to investigate the potential protective role of the mesenchymal stem cells (MSCs) and ischemic pr...

background: proliferation and expansion of cancer stem cells as spheroids were proved in previous studies. but, capability of primary tumor-derived stem cells to keep their unique properties in vitro is still disputed. so, the goal of this study was to isolate, expand and characterize of colon cancer-derived stem cells. material and methods: in the present work, colon cancer stem cells markers ...

Background: CD93 has originally been known as a C1q receptor, and many studies have demonstrated that CD93 is expressed on hematopoietic stem cells, B cell progenitors, myeloid and monocytic cells. Moreover, CD93 is shown to be expressed on long-lived plasma cells, and CD93 deficient-mice display an impairment in plasma cell development. Objective: To investiga...

The appropriate temperature and growth supplementary factors such as fetal bovine serum (FBS) are the necessities needed for cell growth in culture medium. In this regards, the effect of Leibovitz-15 (L-15) medium containing different concentrations of fetal bovine serum (FBS) was assessed on growth and proliferation of cultivated liver cells of klunzingerchr('39')s mullet, Liza klunzingeri, in...

carbosulfan (2,3-dihydro-2,2dimethyl-7-benzofuronyl [(dibutyl amino) thio] methyl] a carbamate insecticide and acaricide was administered orally at an effective dose of 48 mg/kg/day to albino mice for 5, 10, 20 and 30 days .control mice received similar quantities of olive oil. daily body weights were recorded and mice were sacrificed after 24 hours after the terminal exposure. the histologic e...

Background and Objectives: Multiple sclerosis is an inflammatory disease of the central nervous system. This is due to migration of peripherally activated lymphocytes to central nervous system leading to inflammatory lesions. However, liver has an anti-inflammatory microenvironment. Myelin expression in the liver of transgenic mice suppresses inflammatory lesions within central nervous system. ...

P. vermicola is an opportunistic pathogen of human and animals. To estimate the pathogeneses P.vermicola in mice infected orally I/P with 1x108 CFU/ml).The histopathological changes at 24 hrs showed infiltration inflammatory cells apoptosis hepatocytes liver, around a congested blood vessel interstitial tissue kidney; lung revealed congestion vessels, alveolar emphysema; intestine appeared mono...