AIMS The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes. METHODS AND RESULTS IMS-SA tests detected 132 isolates of presumptive...

b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected w...

dna methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. to assess dna methylation pattern in different developmental stages of aeluropus littoralis, methylation sensitive amplified polymorphism (msap) was used. methylation and demethylation status at the ccgg recognition site were tracked ...

background: the aim of this study was dna extraction from protosco-lecses of echinococcus granulosus and identification of these strains in west- azerbaijan province, north western iran. methods: thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. to investigate the genetic variation of the isolates, after dna extraction by glass beads-phenol chlor...

A new approach to characterize growing microorganisms in environmental samples based on labeling microbial DNA with H2 O is described. To test if sufficient amounts of O could be incorporated into DNA to use water as a labeling substrate for stable isotope probing, Escherichia coli DNA was labeled by cultivating bacteria in Luria broth with H2 O and labeled DNA was separated from [O]DNA on a ce...

OBJECTIVES Extended-spectrum beta-lactamase (ESBL)-producing non-typhoidal Salmonella (NTS) isolates in Korea were characterized. PATIENTS AND METHODS Five isolates of ESBL-producing NTS were isolated from stool specimens of three infants and two adults with diarrhoea. Two infants acquired the infection in the community, and three other infections were hospital acquired. RESULTS The isolate...

Background: Fenugreek (Trigonella foenum-graecum L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties. Objective: The aim of present study was to investigate the genetic diversity within and between different populations of Trigonella foenum-graecum using DNA ma...

Genotyping of single nucleotide polymorphisms (SNPs) in large populations presents a great challenge, especially if the SNPs are embedded in GC-rich regions, such as the codon 112 SNP in the human apolipoprotein E (apoE). In the present study, we have used immobilized locked nucleic acid (LNA) capture probes combined with LNA-enhancer oligonucleotides to obtain efficient and specific interrogat...

FIG. 1.-Ultraviolet pH 12.15 2 photographs and densitometer tracings of typical pneumococcal DNA bands in CsCl, with reference bands of dAT polymer (1) and M. lysodeikticus DNA (2). Top: pH 12.15; mid. N D die: dAT plus nativeandi heat-enatured pneumopH7 coccal DNA, pH 7; bottom: J native, pH 7. Centrifuged 21 hr at 44,770 rpm, 2 25.00C. The dAT andM. lysodetkticus bands at pH 12 are still rath...