Freezing of ejaculated and epididymal spermatozoa is currently a subject of interest with the purpose of establishing an efficient gene banking model for valuable animals or endangered species. Therefore, the present study evaluated the influence of different storage duration )0, 1.5 and 5 h) at 5 ºC and type of cryoprotectant on freezability of ram epididymal spermatozoa. With increasing the s...

the freezing and thawing process not only is associated with serious damage to sperm such as damage to the plasma membrane and the acrosomal membrane but also changes the membrane permeability to some ions including calcium. also, the generation of oxygen free radicals is increased during the freezing-thawing process. the purpose of this study was to evaluate of the effects of trolox as an anti...

The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nuc...


 Purpose: To investigate the in vitro effects of methanol leaf extract E. hirta (MLEEH) on motility, viability and morphology caprine spermatozoa.
 Methods: The effect MLEEH treatment (1.25, 2.5, 5, 10 20 mg per mL) sperm percentage total progressive abnormalities were evaluated at 1, 5 min post-treatment. Sperm revival test was used to evaluate reversibility incapacitation following...

Several gene expressions are related to the success of spermatogenesis. Protamine plays an important role in sperm DNA packaging, spermatogenesis, and quality. This study aimed isolate RNA from spermatozoa determine expression profiles protamine 1 (PRM1) 2 (PRM2). Six Pasundan bulls aged 5-8 years with a body weight 380 kg-430 kg were used for this study. The classified into group A (70%-79%) B...

Background: Infertility in men can be caused by decreased motility of spermatozoa. Electric cigarette smoke containing nicotine produce free radicals that cause a decrease sperm motility. Purple grape juice which contains flavonoids act as antioxidants increase the Objective: To prove effect administering purple on male Wistar rats exposed to electric smoke.Methods: This research is an experime...

Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa. Six sperm categories of each stallion (n=4) were evaluated for motility, morphology and viability. These categories were fresh ejaculate...

Background: Many studies are being conducted to improve the quality of bull sperm in storage conditions. It seems that increasing sperm membrane unsaturated fatty acids can enhance sperm quality. This study was aimed to investigate the effects of combination of omega- 3, 6, 9 fatty acids on characteristics of bovine chilled semen. Materials and Methods: Different levels of oil containing omega-...

Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected of having male reproductive toxicity. However, FA male reproductive toxicity was inconclusive due to dearth of human studies. Therefore, we sought to investigate whether occupational exposure to FA affects semen quality. Semen quality including five conventional parameters and seven kinematics parameters were compa...