A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot prim...

Genetically modified crops which had been commercial applied extensively majorly are the insect resistance and herbicide tolerance events. In this study, the Bt insecticidal gene Cry1Ab, the glyphosate-tolerant gene EPSPS, and the selection marker gene PMI were combined into a single transferred DNA fragment and introduced into sugarcane by Agrobacterium-mediated transformation. Thirty-three re...

In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26 degrees C) and low (4 degrees C) temper...

Grapevine (Vitis vinifera L.) is rich in phenylpropanoid compounds, namely flavonoids and stilbenes which, present in most tissues, are described as antioxidants and known to accumulate in response to biotic and abiotic stress. Grapevine is then a choice model for studying the interplay between the phenylpropanoid pathway and nutrient deficiency. Here we report the response to sulfur deficiency...

Abstract Lavender plants are preferably propagated vegetatively. The aim of the work was to propose composition nutrient media for in vitro multiplication Lavandula angustifolia (var. Krajová and Beta) x intermedia Budrovka Grosso). Explant cultures were initiated from apical or nodal segments. Apical segments more suitable regenerated highest number shoots MS medium supplemented with 1 mg/L BA...

Abstract. Harahap F, Nusyirwan N, Afiva A, Prasetya E, Suriani C, Hasibuan RFM, Poerwanto R. 2022. Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers. Biodiversitas 23: 5623-5628. Sipahutar Indonesia, is considered difficult to breed, resulting a lack seed availability. However, tissue cu...

Abstract Shallot ( Allium ascolonicum L), belonging to the genus are propagated vegetatively through layered bulbs. This research aimed improve quality of shallots by eliminating OYDV, SLDV viruses meristematic tissue combined with antiviral Ribavirin. The was conducted in IVEGRI Tissue Culture Laboratory from April August 2018. Research materials included infected shallot bulbs Bima Brebes var...

A novel micropropagation method for pineapple (Ananas comosus L.), based on shoot elongation induced in vitro, was demonstrated for two cultivars. Decapitated in vitro plantlets were used as explants. Shoot etiolation was induced by placing explants in a Murashige and Skoog (MS) medium containing NAA (10 μM) and incubating in darkness at 28C for 30 to 40 days. The mean number of the regenerated...

A living mulch system can provide beneficial biodiversified phytocoenoses and spatial competition against weeds; however, it may also compete for water with the main cultivated crop under Mediterranean climate conditions. Strawberries employed as in a rain-fed hill vineyard of central Italy were evaluated two years through participative approach involving farmer. local wild strawberry was propa...

The present study was undertaken to investigate the role of banana extract (BE) on synchronous plantlet formation from seeds of Dendrobium lituiflorum Lindl. The seedlings formed on modified Knudson C (KC) medium supplemented with 20% (v/v) BE had significantly high fresh and dry weights. Morphologically uniform plantlets with elongated leaves and well developed roots were formed on KC medium w...