the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...

Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns, suggesting that different serotypes have evolved alternative protease cleavage sites. In contra...

As an important industrial enzyme, protease is widely used in feed, food and other fields. At present, the insufficient activity obtained from microorganisms cannot meet purpose of production. In this study, Bacillus amyloliquefaciens with high production was screened animal feces by plate transparent circle method. To improve protease, atmospheric room temperature plasma (ARTP) mutagenesis fir...

Proteolytic enzymes are key signaling molecules in both normal physiological processes and various diseases. After synthesis, protease activity is tightly controlled. Consequently, levels of protease messenger RNA and protein often are not good indicators of total protease activity. To more accurately assign function to new proteases, investigators require methods that can be used to detect and...

We have recently identified in serum an acid protease which is capable of generating des(1-3)IGF-I from intact IGF-I. Here we have utilized a synthetic substrate with the sequence, biotin-G-P-E-T-L-C-BSA which contains the N-terminal sequence of IGF-I, to investigate the levels of this protease activity in streptozotocin-diabetic rats. Protease activity, quantified in terms of the amount of the...

Zn 2+ binds to the active site of SARS-CoV-2 main protease (Mpro), and inhibits enzyme activity viral replication in vitro .

A new protease was found in mitochondria of bone marrow cells. The protease was purified from human bone marrow cells by using the following methods: disruption of cells by sonication; buffer extraction; column chromatography using DEAE-cellulose, Sephadex G-75, and CM-cellulose. It was then crystallized in the presence of polyethylene glycol. The crystallized enzyme is homogeneous as judged by...

A protease active with N-alpha-benzoyl-DL-arginine-p-nitroanilide with an optimum pH of 7.3 has been found in the cytosol of rat liver. The activity of this protease increased in N-2-fluorenylacetamide-induced hepatoma as well as in fetal liver. It has been purified from normal liver and hepatoma about 200-fold. Its molecular weight is estimated by gel filtration to be about 200,000 in each tis...