Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...

Crystalline surface layer proteins (S-layer proteins) have considerable potential for the crystalline arrays in biotechnology, biomimetics and nonlife applications, including areas such as microelectronics and molecular nanotechnology. The extensive application potential of surface layers in nanobiotechnology is according to the particular inherent attributes of the single molecular arrays cons...

This paper summarizes R&D activities and progress at NORAM Engineering and the University of British Columbia (UBC) on preparation and testing of thin palladium-based membranes and their applications. Most of these activities were carried out internally at NORAM, some jointly with UBC and their spin-off company, Membrane Reactor Technology (MRT) through a wide range of projects. Key results out...

objective: liver is an important organ that is exposed to many oxidant and carcinogenic agents, thus antioxidant compounds are beneficial for liver health. artemisia contains flavonoid compounds and anti-diabetic, antioxidant, and anti-inflammatory properties. due to possessing terpene and sesquiterpene compounds, this plant has antioxidant properties. this study was done to investigate the eff...

Introduction 2 Deoxyribonucleic Acid to Ribonucleic Acid 4 Ribonucleic Acid to Protein 6 Proteome Handling in Classical and Functional Proteomics 6 Protein Purification 6 Cell Culture 8 Other Source of Samples 8 Subpopulation of Cells 10 Subcellular Components 11 Protein Separation by Two-Dimensional Gel Electrophoresis 12 Principles of Two-Dimensional Gel Electrophoretic Separation 13 Detectio...

Phase separation is a simple, efficient, and cheap method to purify and concentrate detergent-solubilized membrane proteins. In spite of this, phase separation is not widely used or even known among membrane protein scientists, and ready-to-use protocols are available for only relatively few detergent/membrane protein combinations. Here, we summarize the physical and chemical parameters that in...

There has been much interest generated in the recovery of nanoparticulate (nanoparticle) bioproducts(Second generation of biotechnological products) such as plasmid DNA and viruses as putative gene therapyvectors, macromolecular assemblies as drug delivery vehicles and virus-like particles as vaccine components.Such product must be manufactured in advanced stages of purity, ma...

Tampereen ammattikorkeakoulu Tampere University of Applied Sciences Paper, Textile and Chemical Engineering Arina Akhmetova: Comparison of ultrafiltration and size-exclusion chromatography performances for proteins desalination Bachelor's thesis 41 pages November 2014 Protein purification is a part of biotechnology. Its application can vary from laboratory researches to industrial production. N...