To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm diameter) Sw. derived from MS supplemented with 0.5 mg/L TDZ 60 days culture were cultured on 6 media; Murashige Skoog (MS) medium, 15 % (v/v) CW, CW 0.2 (w/v) AC, Vacin Went (VW) VW AC. After months culture, containing coco...

Abstract Background Dendrobium officinale , an endangered Chinese herb, possesses extensive therapeutic effects and contains bioactive ingredients such as major polysaccharides, alkaloids, minimal flavonoids. We first obtained the protocorm-like bodies (PLBs) of this plant through tissue culture in order to determine distribution main secondary metabolites each organelle PLBs. then analyzed cor...