The construction of high-yield laccase-producing fusant was achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions demonstrated that with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened by different characters between two parental strai...

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...

Pea protoplasts dielectrophoretic coefficients were measured in alternating electric fields of frequency 1 MHz and voltage 5 V applied between two concentric cylindrical metal electrodes of outer and inner radii 0.24 mm and 1 mm, respectively. They do not vary significantly with solution osmolarity, but* show a clear expressed maximum in isotonic conditions; the values in 0.4 m , 0.5 m , 0.6 m ...

Shockman, Gerald D. (Temple University School of Medicine, Philadelphia, Pa.), Joseph J. Kolb, Bohdan Bakay, Margaret J. Conover, and Gerrit Toennies. Protoplast membrane of Streptococcus faecalis. J. Bacteriol. 85:168-176. 1963.-The membrane fraction of Streptococcus faecalis (ATCC 9790) was isolated and purified, by a variety of procedures, from cultures that were grown under closely controll...

The inhibitory allelopathic activity of Pueraria montana (Kudzu), and activities two putative allelochemical isoflavones, puerarin daidzein, were evaluated using the protoplast co-culture method with digital image analysis lettuce as a recipient (DIA-PP method). Cotyledon protoplasts Kudzu isolated Cellulase R10 Driselase 20 in 0.6 M mannitol solution. Optimal hormonal condition density for gro...

Stable protoplasts may be released from MiCrococcua Zysodeikticus and Sarcina Zutea by digestion of the cell-wall with lysozyme in sucrose or NaCl solutions having an osmotic pressure of some 25 atmospheres, but not in glycerol solutions of the same osmotic pressure. The stability of the protoplasts depends not only upon the depression of the water activity by the solute but upon an osmotic pre...

The date palm has a pivotal role in the oasis ecosystem and it is an important food crop and commercial commodity. The cloning or protoplast fusion can increase breeding and multiplication of many varieties. Present investigation has been undertaken with the aim to develop methods for obtaining plantlets by somatic embryogenesis either from vegetative or female floral explants, as well as by th...