AvrPtoB is a type III effector protein from Pseudomonas syringae pv. tomato that physically interacts with the tomato Pto kinase and, depending on the host genotype, either elicits or suppresses programmed cell death associated with plant immunity. We reported previously that avrPtoB-related sequences are present in diverse gram-negative phytopathogenic bacteria. Here we describe characterizati...

Small non-coding RNAs (ncRNAs) are important components of many regulatory pathways in bacteria and play key roles in regulating factors important for virulence. Carbon catabolite repression control is modulated by small RNAs (crcZ or crcZ and crcY) in Pseudomonas aeruginosa and Pseudomonas putida. In this study, we demonstrate that expression of crcZ and crcX (formerly designated psr1 and psr2...

Aim. For the purpose of correct species and internal identification isolated strains bacteria that cause walnut leaf spotting, molecular biological genetic detection their ability to synthesize phytotoxin syringomycin have been carried out. Methods. It has used microbiological (PCR) methods. Results. The presence (syrD) secretion genes was established in spot pathogen typical strain Pseudomonas...

Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcribed spacer, ITS1. No difference among all strains in rrs and rrl genes was observed with 14 rest...

In this study, for the first time, functionally active, recombinant, cysteine-rich plant proteins snakin-1 (SN1) and defensin (PTH1) were expressed and purified using a prokaryotic expression system. The overall level of antimicrobial activities of SN1 and PTH1 produced in Escherichia coli was commensurate with that of the same proteins previously obtained from plant tissues. Both proteins exhi...

The present study is to concentrate on the synergistic bactericidal effect of silver nanoparticles and ciproflaxacin against Pseudomonas solanocearum, Pseudomonas syringae, Xanthomonas malvacearum and Xanthomonas campestris. Silver nanoparticles were prepared in 0.1mM, 0.2 mM and 0.3 mM by Spirulina platensis. It exhibited anti microbial activity against all phytopathogens with varying degrees....

Plant-associated pseudomonads are commonly exposed to copper bactericides, which are applied to reduce the disease incidence caused by these bacteria. Consequently, many of these bacteria have acquired resistance or tolerance to copper salts. We recently conducted a survey of 37 copper-resistant (Cur) Pseudomonas spp., including P. cepacia, P. fluorescens, P. syringae, and P. viridiflava, and f...