BACKGROUND There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expression of LACK gene, a 36kD protein, as a candidate protein for vaccination against Iranian L. infantum. METHODS Ira...

BACKGROUND Heat shock protein 70 (HSP70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including Leishmania spp. OBJECTIVE The aim of this study was to clone and express HSP70 from L. infantum strain MCAN/IR/96/LON-49 and evaluate antibody response against HSP70 in visceral leishmaniasis (VL). METHODS The L. infantum HSP70 gene segmen...

introduction bovine ephemeral fever (bef) is an arthropod-borne viral disease of cattle and water buffalo, spanning tropical and subtropical zones in asia, australia, and africa continents. the clinical signs of bef disease in cattle are acute febrile reaction, stiffness, lameness, depression, cessation of rumination, and constipation. this disease was caused by ephemerovirus of the rhabdovirid...

OBJECTIVE To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine. METHODS The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable...

conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...

Previous studies of antibody responses to Mycobacterium tuberculosis antigens at different stages of tuberculosis (TB) and in different classes of TB patients provided evidence that the profile of antigens recognized by antibodies is altered with disease progression. M. tuberculosis is a slow-growing organism, and it takes several weeks to months for an infection to progress to clinical TB. App...

BACKGROUND Although the development of novel therapeutic regimens to combat hepatitis C virus (HCV) infection have been speeded up with successful results, no efficient vaccines exist yet. OBJECTIVES This study aimed to construct a eukaryotic expression vector encoding nonstructural proteins, NS3/NS4A, of HCV genotype 3a, and evaluate its expression on Huh7 cell surface. MATERIALS AND METHO...