phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

background: taq dna polymerase is a very important enzyme for molecular biological studies such as dna amplification and dna sequencing by the pcr. it is a standard enzyme that is used in 90% of molecular biology labs today. the aim of this study was to produce taq dna polymerase enzyme in e. coli by a reliable, practical, simple and low cost method.materials and methods: in this study, the taq...

conclusions accidents resulting in death, total disability, and partial disability impose a huge cost burden on society, whereas the employer will bear a relatively low cost. this is because of society’s health, treatment, and welfare systems available for treatment and rehabilitation of injured employees and their families. background occupational accidents represent one of the major problems ...

microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. in addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. bacillus sp. dm12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...

Genetic engineering enables the construction of gene man Genome Project (5), efficient and robust production and purification strategies are necessary (6). For fusions resulting in fusion proteins having the combined properties of the original gene products. Fusion industrial production of recombinant proteins, simple and fast purification methods introduced as an early can be made on either or...

background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. there­fore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...

BACKGROUND Concentration and purification of ethanol and other biofuels from fermentations are energy-intensive processes, with amplified costs at smaller scales. To circumvent the need for these processes, and to potentially reduce transportation costs as well, we have previously investigated bio-hybrid fuel cells (FCs), in which a fermentation and FC are closely coupled. However, long-term op...