"Barcode-tagged" PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyro...

Prostate cancer is the second major cause of male deaths. Obesity, type 2 diabetes, and risk are linked. Insulin-like growth factor II (IGF-II) involved in numerous cellular events, including proliferation survival. The IGF-II gene shares its locus with lncRNA, H19. IGF-II/H19 was first to be identified as being “imprinted”—where paternal copy not transcribed—a silencing phenomenon lost many ty...

BACKGROUND 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, e...