*Corresponding Author: Dr. Srinivas. C, Department of Microbiology and Biotechnology, Bangalore University, Jnanabharathi Campus, Bangalore-560 056, Karnataka, India. Page| 342 Efficacy of Trichoderma asperellum against Ralstonia solanacearum under greenhouse conditions Narasimha Murthy K, Nirmala Devi D and Srinivas C* Department of Microbiology and Biotechnology, Bangalore University, Jnanabh...

Ralstonia solanacearum displays variability in its virulence to solanaceous crops. We report here the draft genome sequences of eight phylotype I strains and one phylotype III strain differing in virulence to the resistant eggplant genotype AG91-25. These data will allow the identification of virulence- and avirulence-related genes.

PhiRSB1 is a wide-host-range, T7-like bacteriophage that infects and efficiently lyses the phytopathogenic bacterium Ralstonia solanacearum. The phiRSB1 genome comprises 43,079 bp of double-stranded DNA (61.7% G+C) with 325-bp terminal repeats and contains 47 open reading frames. Strong activity of tandem early promoters and wide specificity of phage promoters of phiRSB1 were demonstrated.

Genes homologous to avrBs3 of Xanthomonas were detected in 309 strains of Ralstonia solanacearum biovars 3, 4, and 5 but not biovar 1 or 2. A statistically significant association between the originating plant species and internal repeats of the gene was found. Sequences of repeats and variation between nearly clonal strains revealed evidence of frequent recombination.

UNLABELLED During bacterial wilt of tomato, the plant pathogen Ralstonia solanacearum upregulates expression of popS, which encodes a type III-secreted effector in the AvrE family. PopS is a core effector present in all sequenced strains in the R. solanacearum species complex. The phylogeny of popS mirrors that of the species complex as a whole, suggesting that this is an ancient, vertically in...

Bacterial wilt is one of the most severe tomato diseases worldwide and Ralstonia solanacearum is determined to be the pathogen responsible for its spread. Soil amendments such as calcium cyanamide and herb residues have both been proved to be high potential for tomato bacterial wilt control. Disinfection effect and heat generated during the decomposition processes of calcium cyanamide and herb ...

Bacterial wilt is one of the most important crop diseases, and is caused by the soil-borne Gram-negative bacterium Ralstonia solanacearum. R. solanacearum was formerly classified as Pseudomonas solanacearum or Bacterium solanacearum (Smith, 1986; Yabuuchi et al., 1995). This bacterium has an unusually wide host range, infecting more than 200 species belonging to more than 50 botanical families,...

Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or un...

Pathogenicity of Xanthomonas and most other Gram-negative phytopathogenic bacteria depends on a conserved type III secretion (T3S) system which injects more than 25 different effector proteins into the plant cell. Extensive studies in the last years on the molecular mechanisms of type III effector function revealed that effector proteins with enzymatic functions seem to play important roles in ...

Ralstonia solanacearum is a well-known agricultural and ecological threat worldwide. The complexity of the R. solanacearum species complex (Rssc) represents a challenge for the accurate characterization of epidemiological strains by official services and research laboratories. The majority of protocols only focus on a narrow range of strains; however, this species complex includes strains that ...