BACKGROUND Real-time quantitative reverse transcription PCR (RQ-PCR) assay for BCR-ABL is used to monitor treatment response in chronic myeloid leukemia (CML). BCR-ABL transcript levels decline over several years of imatinib treatment, and increasing numbers of patients have BCR-ABL transcripts at or below the limit of detection. More sensitive PCR methods are required to assess whether these p...

RAPD is a simple dominant marker system widely used in biology. Effectiveness of RAPD can be improved by selecting and redesigning primers whose priming sites occur in target sequence(s) of gene or organism at optimum distance. We developed software that uses sequences of random decamer primers and nucleotide sequence(s) as two input files. It locates the priming sites in input sequences and ge...

leaf blotch disease of barley, caused by rhynchosporium secalis is a major disease of barley in iran. its worldwide occurrence and economic importance in barley production has motivated studies on the population genetic structure of this pathogen. random amplified polymorphic dna (rapd) method was utilized to investigate the genetic diversity of populations of r. secalis isolated from barley le...

Beginning in 2001, the Millennium Cohort began enrollment of a stratified random sample of more than 140,000 service members, with the intention of following their health for up to 20 years. Cohort members will provide self-reported data on medical symptoms, functional status, and health-related behavior every 3 years. New and existing sources of objective data on health and health-related expo...

Eight geographical isolates of Schistosoma japonicum from Taiwan and mainland China and one isolate of Schistosoma mansoni were studied by RAPD analysis using six arbitrary primers and SSR-PCR analysis using a (CA)8RY primer. The genetic distance was determined by the percentage of unshared bands. The RAPD and SSR-PCR results showed that the genetic distance between S. mansoni and S. japonicum ...