The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, economical method for diagnosis of H. pylori infection in dyspeptic patients.

The presence of C pylori infection was determined in 1445 patients undergoing upper gastrointestinal endoscopy over a 12 month period. The presence of C pylori was detected in gastric mucosal biopsy specimens by the biopsy urease test, microscopy (Gram stained smears and histology) and culture. Two media were used for the biopsy urease test: Christensen's urea broth (for the first 600 patients)...

INTRODUCTION The HpOne test is a new five-minute rapid urease test developed for the rapid detection of Helicobacter pylori infection during gastroscopy. However, evidence in the literature supporting its use clinically is scarce. The most commonly used rapid urease test remains the Campylobacter-like organism (CLO) test, which generates accurate readings only after 24 hours. The aim of this st...

Helicobacter pylori (Hp) were detected in the dental plaque in 40 patients with peptic ulcer, chronic gastritis or gastric cancer with rapid urease test, anti-Hp fluorescein-labelled antibody staining, bacterial culture and electronic microscopy. At the same time, biopsy specimens from the gastric antrum of these patients were studied with WS staining and rapid urease test to detect Hp. The res...

BACKGROUND The purpose of this study was to evaluate prospectively the presence and impact of the gastric carcinogen Helicobacter pylori (H. pylori) in the upper aerodigestive tract. Previous studies suggested it could represent a risk factor for head and neck squamous cell carcinoma (HNSCC). METHODS Serology, rapid urease test, and quantitative polymerase chain reaction (qPCR) for H. pylori ...

BACKGROUND/AIMS To evaluate a new monoclonal antibody for Helicobacter pylori urease in gastric tissue. METHODS A total of 107 volunteers were enrolled. All subjects underwent a (13)C-urea breath test and esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia. Six biopsy specimens in the gastric antrum and body were obtained for a rapid urease test and histology. The n...

conclusion it was concluded that elisa-based anti h. pylori igg test in saliva could be used as an alternative diagnostic test in the absence of other invasive procedures. background helicobacter pylori is known as an agent which may involve in the occurrence of peptic ulcer, gastric cancer and also other known and unknown diseases. treatment of the infection with antibiotics eradicates the dis...

Three rapid methods (spot test, disk, and tube) for detecting urease production in anaerobic bacteria yielded results faster than the conventional method. The results were more consistent with the disk and tube methods than with the spot test. Blood agar plate growth gave more consistent results than growth from chopped-meat slants.