The prolactin secretory activity of anterior pituitary during estrous cycle was determined from 14C-leucine incorporation in vitro into the hormone, using IVCS mice which showed regular 4-day-cycles. Prolactin in the anterior pituitary and in the incubation medium was fractionated by disc electrophoresis on polyacrylamide gel. The synthesis and release of prolactin were significantly lower at e...

Wnt signaling is important in many aspects of cell biology and development. In the mouse female reproductive tract, Wnt4, Wnt5a, and Wnt7a show differential expression during the estrus cycle, suggesting that they participate in female reproductive physiology. Although the pituitary is a major gland regulating reproduction, the molecular mechanism of Wnt signaling here is unclear. We elucidated...

Blood serum progesterone (P4) levels in donor cows and heifers at donor selection, superovulation, insemination and embryo recovery were investigated. Significant differences in P4 levels in the investigated donors during superovulatory oestrus insemination were observed. The cows with low levels of P4 at insemination (5.8 ± 1.7 nmol/l) showed a better yield and quality of embryos (P<0.01) comp...

Levels of luteinizing hormone (LH), prolactin, progesterone, cortisol, estrone and estradiol-17~ were determined during the postpartum anestrous period in samples collected from six second-calf, crossbred, suckled beef cows. Blood samples were collected at 6-h intervals beginning 2 wk before expected parturition and continuing until 10 d after first postpartum estrus. Samples were also collecte...

Multiparous Brahman cows were assigned by order of calving and sex of calf to groups to be fed to maintain body condition score (BCS) of 6 or greater (M; n = 10) or to lose BCS (L; n = 10). Blood samples were collected weekly for progesterone analysis and at 15, 30 and 45 d after parturition at 15-min intervals for 6 h for determination of immunological (ILH) and biological (BLH) luteinizing ho...

the aim of the study was to investigate the preventive effects of dietary garcinia cambogia extract onlipid metabolism and serum activities of alanine aminotransferase (alt), aspartate aminotransferase (ast)and gamma-glutamyle transferase (ggt) in rats fed high-lipid diet. thirty female, one-year-old sprague-dawley rats were used and separated into three equal groups. group 1 (control group) wa...

Antiserum generated in a horse against testosterone conjugated to bovine serum albumin (BSA) was administered to six lighthorse mares (androgen-immunized mares) 1 to 3 d before a prostaglandin-induced estrus and twice again at 2-d intervals. Six control mares were administered antiserum generated against BSA on the same schedule. Relative to testosterone, cross-reactivities of other steroids wi...

Ovarian dysfunction leading to hormonal imbalance plays a crucial role in uterine carcinogenesis in rats as well as women. However, the effects of a reduction in primordial follicles at birth on uterine adenocarcinoma development have hitherto not been determined. The present study was therefore conducted using female Donryu rats, a high incidence rat strain of uterine adenocarcinoma. The anima...

Guanosine 3',5'-cyclic monophosphate (cGMP), as a second messenger, plays potential roles in ovarian functions. To elucidate the role of phosphodiesterase (PDE) in cGMP signaling during ovarian follicular development, the present study was conducted to investigate ovarian cGMP level and cGMP-PDE activity by radioimmunoassay (RIA) in postnatal rats, immature rats during gonadotropin-primed folli...

A study utilizing 64 Angus and Simmental multiparous beef cows was conducted to evaluate the administration of MGA-PGF on the onset of estrus, conception rate, and to determine blood serum hormone profiles (P, & LH). Cows utilized were at least 30 days postpartum and stratified by age, breed, and days postpartum into two groups: a treatment group (n=32) which received MGA orally for 14 days and...