Work by Marinetti, Erbland & Stotz (1958) on pig-heart-cell fractions and by Macfarlane, Gray & Wheeldon (1960) on rat-liver-cell fractions have indicated that the mitochondrial and microsomal phospholipids may differ significantly in composition. In view of the results obtained by Collins (1960) and by Collins & Shotlander (1961), showing the widespread occurrence of complex amino-phospholipid...

and staining properties characteristic of normal intracellular liver mitochondnia (8). The method consists essentially in counting fresh mitochondria dispersed at random in a known volume of suspension, and it can be applied to the study of the number of mitochondria per normal rat liver cell. Preliminary results are here with reported for the liver cell in other physiologi cal and pathological...

1. The mechanism of sulphite and sulphate permeation into rat liver mitochondria was investigated. 2. Extramitochondrial sulphite and sulphate elicit efflux of intramitochondrial phosphate, malate, succinate and malonate. The sulphate-dependent effluxes and the sulphite-dependent efflux of dicarboxylate anions are inhibited by butylmalonate, phenylsuccinate and mersalyl. Inhibition of the phosp...

The effect of thiamine and its various derivatives on the respiratory control of rat liver mitochondria was examined. Except TDP all other thiamine derivatives tested were shown to damage mitochondrial respiration. In these experiments DCET and T A TD are shown to be strong inhibitors. It was noted that thiamine and its derivatives except TDP have aldehyde group in their chemical structure. Bec...

1. Phenethylbiguanide inhibits the synthesis of phosphoenolpyruvate from malate or 2-oxoglutarate by isolated guinea-pig liver mitochondria. This inhibition is time- and concentration-dependent, with the maximum decrease in the rate of phosphoenolpyruvate synthesis (80%) evident after 10min of incubation with 1mm-phenethylbiguanide. 2. The phosphorylation of ADP by these mitochondria is also in...

The relationship between citrate and mitochondria was the subject of earlier papers of Potter (I), Meduski (2), Harman and Feigelson (3), and Plaut and Plaut (4), all of them reporting the lack of citrate oxidation by rat (l), pig (2), rabbit (3), and guinea pig (4) heart preparations. Retention of endogenous citrate by intact isolated mitochondria was demonstrated by Schneider, Striebich, and ...

      Doxorubicin (DOX) is an anthracycline antibiotic that has been used for a long time in therapy of an array of human malignancies either alone or in combination with other cytotoxic agents. The dose-dependent cardiotoxicity of DOX significantly limits its anticancer efficacies. Oxidative stress caused by enhanced production of reactive oxygen species is an important contributor to DOX mito...

1. Carnitine acetyltransferase (EC 2.3.1.7) activity in sheep liver mitochondria was 76nmol/min per mg of protein, in contrast with 1.7 for rat liver mitochondria. The activity in bovine liver mitochondria was comparable with that of sheep liver mitochondria. Carnitine palmitoyltransferase activity was the same in both sheep and rat liver mitochondria. 2. The [free carnitine]/[acetylcarnitine] ...

not received Giutamate transport inhibitor Glutamate oxidation Rat liver mitochondria