Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...

In a choice reaction time (RT) task, electromyographic (EMG) recordings allowed us to fractionate RT into two subcomponents, namely premotor time and motor time. This has been done for correct trials and errors. The analysis of the EMG burst and motor time (between EMG onset and overt response) showed that the EMG burst amplitude was reduced and the motor time was longer for errors than for cor...

UNLABELLED This research note describes potential trends in the reaction time (RT) performance of persons who stutter (PWS). The main purpose of this note is to describe these trends to researchers, encourage further research in this area, and alert researchers to possible concerns about the interaction of certain reaction time research procedures and characteristics of PWS. Post hoc analyses a...

In this article, the development of a new TaqMan-based one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection and quantification of Crimean-Congo hemorrhagic fever virus (CCHFV) RNA is described. Selected oligos targeting the highly conserved S region of CCHFV were designed by using our oligo design and analysis software, Oligoware 1.0. None of the prime...

A real-time reverse-transcription polymerase chain reaction (RT-PCR) was developed for efficient detection of genetically diverse Pepino mosaic virus (PepMV) isolates. The novel detection system was designed to use a duo-primer system targeting the conserved region in the triple gene block 2 (TGB2) gene with a single conserved TaqMan probe to broaden its reaction to cover all available PepMV st...

Intraindividual variability of reaction time (IIVRT), a proposed cognitive marker of neurobiological disturbance, increases in old age, and has been associated with dementia and mortality. The extent to which IIVRT is an independent predictor of mortality, however, is unclear. This study investigated the association of IIVRT and all-cause mortality while accounting for cognitive level, incident...

The relative contributions of chromatic and achromatic activity to reaction time were investigated under conditions in which spatial and temporal transients were manipulated. Simple reaction times (RT) were obtained to eight photometrically matched (I cd, m:) wavelengths between 448 and 658 nm. These stimuli were incrementally presented on a spatially coextensive 1.2’ white adapting field. RT w...

The authors examined possible differences in left- and right-handers on bimanual reaction times to centralized visual stimuli. Eighty participants (n = 40 in each group of left- and right-handers) were tested on unimanual and bimanual reaction time (RT) tasks. Consistently across the 2 groups, the dominant-hand RT was faster, on average, than the nondominant-hand RT, and unimanual RTs were fast...

The reverse transcription polymerase chain reaction (RT-PCR), reverse passive haemagglutination assay (RPHA) and agar gel precipitation test (AGPT) were compared for the detection of infectious bursal disease virus (IBDV) in various organs of experimentally infected broilers. RT-PCR and RPHA required a time of 8 and 1 hour, respectively, whereas AGPT showed precipitation reaction at least after...