BACKGROUND Protein profiling with high-throughput sample preparation and MALDI-TOF MS analysis is a new potential tool for diagnosis of human diseases. However, analytical reproducibility is a significant challenge in MALDI protein profiling. This minireview summarizes studies of reproducibility of MALDI protein profiling and current approaches to improve its analytical performance. METHODS T...

MALDI MS profiling, using easily available body fluids such as blood serum, has attracted considerable interest for its potential in clinical applications. Despite the numerous reports on MALDI MS profiling of human serum, there is only scarce information on the identity of the species making up these profiles, particularly in the mass range of larger peptides. Here, we provide a list of more t...

In the interest of a more thorough understanding of the relationship between sample deposition technique and the quality of data obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, details of the electrospray (ES) process of sample deposition are investigated using a number of techniques. Sample morphology was observed with scanning electron microscopy (SEM) an...

BACKGROUND Pelvic organ prolapse (POP) is a major health problem in adult women that involves many factors. No proteomic analysis has been conducted exclusively in POP patients. This study aimed to identify the differential expression of proteins that may be involved in POP by proteomic analysis. METHODS Samples of the uterosacral ligament (USL) were collected from five POP patients and five ...

食品由来の酵母19菌種75株についてポテトデキストロース寒天培地, 25 °C，5日間培養した後, VITEK MSTM plusを用いてマススペクトルを取得し, SuperSpectrum (SSp) を作成した. 作成したSSpについて検証を行った結果, 同定の信頼性レベルはSchizosaccharomyces 属, Sterigmatomyces elviae, Starmerella apicola, S. etchellsii, Wickerhamomyces subpelliculosus, Zygosaccharomyces rouxii，Zygosaccharomyces bisporus では80 %以上であった. Z. bailii，Z. parabailii，Z. pseudobailii，Z. mellis, およびZ. siamensisは, それぞれのS...

Reported matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification rates of Gram-positive rods (GPR) are low compared to identification rates of Gram-positive cocci. In this study, three sample preparation methods were compared for MALDI-TOF MS identification of 190 well-characterized GPR strains: direct transfer, direct transfer-formic acid prep...

In our group, we perform successfully MALDI by using femtosecond laser techniques in order to examine the ionization process. One recent result is MALDI with ultrashort laser pulses in the near IR (central wavelength of =800 nm) [2]. In this wavelength regime, used matrix substances have no absorption bands. Experiments with different laser pulse durations suggest that multi photon ionization i...

Since its introduction during the last decade, MALDI mass spectrometry imaging (MSI) is now a routine technique in biology. Nevertheless, a missing link exists in MALDI MSI. Lipids, peptides/proteins, metabolites and drugs can easily be mapped using MALDI-MSI, but this technique has not yet been used to map the transcriptome, which includes microRNA, siRNA and other components. This latter fiel...