PURPOSE To optimize and streamline molecular genetics techniques in diagnosing choroideremia (CHM). METHODS PCR primers were designed for exons 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and 15 of the CHM gene. Each PCR protocol was optimized so that all exons could be amplified with the same component ratio and PCR conditions. Sense and antisense primers were tested for their ability to be used as seq...

The rep-PCR DNA fingerprint technique using BOX A1R primer was investigated. DNA fingerprints were generated from Escherichia coli strains isolated from human, duck, goose, gull, and heron sources. Optimization tools (use of restriction enzymes, BSA, DMSO, and storage buffer) were used in an attempt to generate clear, well-defined DNA fingerprints from the Escherichia coli isolates. Initial stu...

A total of 119 Bacillus thuringiensis strains (83 type strains and 26 native isolates), as well as five B. cereus group species, were analyzed by repetitive extragenic palindromic sequence-based PCR analysis (Rep-PCR) fingerprinting. Primers Bc-REP-1 and Bc-REP-2 were specifically designed according to an extragenic 26-bp repeated sequence found in the six B. cereus group genomes reported. A to...

Previous studies have demonstrated that the four subspecies of the human pathogen Francisella tularensis, despite showing marked variations in their virulence for mammals and originating from different regions in the Northern Hemisphere, display a very close phylogenetic relationship. This property has hampered the development of generally applicable typing methods. To overcome this problem, we...

Despite efforts to minimize fecal input into waterways, this kind of pollution continues to be a problem due to an inability to reliably identify nonpoint sources. Our objective was to find candidate source-specific Escherichia coli fingerprints as potential genotypic markers for raw sewage, horses, dogs, gulls, and cows. We evaluated 16S-23S rRNA intergenic spacer region (ISR)-PCR and repetiti...

alder (alnus subcordata subsp. subcordata) is one of the most important tree species in northern forests of iran and is economically considered as the fourth commercial tree. a leaf spot disease of alder caused by xanthomonas sp. has been reported from several forest regions of mazandaran province (rahimian et al. 1383. 16th iran. plant protec. cong., 439). according to a recent genomic study, ...

Adeno-associated virus (AAV) is a human parvovirus that normally requires a helper virus such as adenovirus (Ad) for replication. The four AAV replication proteins (Rep78, Rep68, Rep52, and Rep40) are pleiotropic effectors of virus integration, replication, transcription, and virion assembly. These proteins exert effects on Ad gene expression and replication. In transient plasmid transfection a...

Xanthomonas campestris pv. (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agroecological conditions. The bacterium survives soil, plant residues, seeds, and wild weedy plants. This study aimed to characterize Xcc isolates that persist field soil roots where they represent risk causing disease Collection samples was carried out period 2015 - 2017 four locations re...