Two polymorphic marker genes, merozoite surface protein 3α (PvMSP3α) and merozoite surface protein 3β (PvMSP3β), from 100 Plasmodium vivax field isolates, were investigated using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). Genotyping of PvMSP3α and PvMSP3β revealed marked polymorphisms in length and sequence. Three major types of PvMSP3α (Type A, B and C) ...

Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by enzymes. RFLP-PCR an easy-to-perform and inexpensive tool for initial analysis SNPs potentially associated with some monogenic diseases, as well in genotyping, genetic mapping, lineage screening, for...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

background: reduced bioavailability of nitric oxide (no) and the t?786c polymorphism of endothelial nitric oxide synthase (enos) gene have been reported as risk factors for the development of coronary artery disease (cad) with conflicting results. we investigated the association of plasma no levels, t?786c genetic polymorphism, and gene expression levels of enos with cad risk in an iranian subp...

Background: Staphylococcus aureus is a common pathogen in human that can be the cause of a wide range of infectious diseases including bacteremia, pneumonia, cellulitis, and osteomyelitis and skin and soft tissue infections. The coagulase enzyme is one of the most important virulence factors of this bacterium. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Coa...

conclusions the results of antifungal susceptibility tests demonstrated that voriconazole was the most active drug against candida isolates. candida albicans is the most common yeast isolated from human blood world-wide; the unexpectedly higher rate of c. parapsilosis isolated in this study necessitates more studies with larger sample sizes in iran. results candida parapsilosis (c. parapsilosis...