We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing platform. Two sets of 85 adapter pairs were developed to concurrently identify SNPs, indels and ...

Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates that P-elements inserted into the transcribed region of y can terminate y trans...

If JR is a ring, and G is a group of automorphisms of R, then R denotes the subring of R consisting of elements of R left fixed by every element of G, and is called the Galois subring corresponding to G. In his paper, Groups acting on hereditary rings, G. M. Bergman has asked if every Galois subring of a right Ore domain corresponding to a finite group is itself right Ore. In this note we show ...

defined by sending E to E|C . It is shown in [4] that Φk is a dominant map to W,W and W , for k = 1, 2, 3, respectively. In this article, we give a proof of the dominance of the rational map Φ4. This is equivalent to the dominance of the rational map from M(3, 6) to SUC(2, 3KC) by twisting. For a general bundle E ∈ SUC(2, 3KC), we embed C with P2 into a Grassmannian Gr(5, 2) and take the pull-b...

A b s t r a c t : We provide a model for reasoning about knowledge anti probability together. We a.llow explicit mention of probabilities in formulas, so that our language has formulas tha.t essentia.lly say "a.ccording to agent i, formula. (p holds with probability a.t least o~." The language is powerfid enough to allow reasoning a~bout higher-order probabilities, as well as allowing explicit ...

HomX denotes the sheaf-Hom functor of OX -complexes: HomX(E,F )(U) := Hom • U (E|U , F |U ) (U ⊂ X open), the restriction map for U ′ ⊂ U being the obvious one. This “dynamic” sheafified version of Hom• has a derived functor RHom•, defined as usual via q-injective resolutions (which always exist!). Similarly, we have a sheaf-theoretic version of ⊗, and its left-derived functor ⊗ = , defined via...

We consider functions f : B → R that obey tropical analogs of classical Plücker relations on minors of a matrix. The most general set B that we deal with in this paper is of the form {x ∈ Z : 0 ≤ x ≤ a, m ≤ x1 + . . . + xn ≤ m ′} (a rectangular integer box “truncated from below and above”). We construct a basis for the set T of tropical Plücker functions on B, a subset B ⊆ B such that the restr...

We have tested several strains of Streptomyces aureofaciens from different sources for restriction endonuclease. In all tested tetracycline producing strains (B-96, NMU, 16, R8/26) restriction endonuclease was isolated and characterized (SauLPl, SauNl, SauSl, SauHPl respectively). Previously described restriction endonuclease from strain S. aureofaciens R8/26I (1) was renamed for SauHPl in this...