We present the design of an object-oriented database system for reverse genetics applications. Such a database will encapsulate not only the data in the genetic and physical maps, but also the methods used to create the maps as well as methods to link them to other databases, such as GenBank, PIR, and MedLine. The purpose of this database is to provide the fungal genetics community with an elec...

Reverse genetics is a gene-driven approach that deduces, from directed mutations, the full range of phenotypes controlled by each gene. This investigative process proceeds in the opposite direction of so-called forward genetic screens of classical genetics. Genome sequencing projects have identified large numbers of organisms’ genes for which no role has yet been defined. To address this proble...

Noroviruses are the major cause of nonbacterial gastroenteritis in humans. These viruses have remained refractory to detailed molecular studies because of the lack of a reverse genetics system coupled to a permissive cell line for targeted genetic manipulation. There is no permissive cell line in which to grow infectious human noroviruses nor an authentic animal model that supports their replic...

Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, ...

TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Dro...