The hologic panther fusion (PF) platform provides fully automated CE marked diagnostics for respiratory viruses, including the recently discovered severe acute syndrome-coronavirus 2 (SARS-CoV-2) by a transcription mediated amplification (TMA) assay, but not endemic human coronaviruses (hCoV). Therefore, laboratory developed test (LDT) comprising multiplexed reverse polymerase chain reaction (R...

One of the viral diseases affecting both domestic and wild animals in many countries around world is foot mouth disease (FMD). The livestock sector has been suffering significant financial losses for years as a result disease. present research work was focused on isolation, documentation serotyping Foot Mouth Disease (FMD) virus from different locations. Reverse Transcription Polymerase Chain R...

OBJECTIVE To identify associations between microbes and host genes in cats with feline chronic gingivostomatitis (FCGS), a debilitating inflammatory oral mucosal disease no known cause, compared healthy periodontitis (control cats). ANIMALS 19 control 23 FCGS. PROCEDURES At least 1 caudal swab specimen was obtained from each cat. Each underwent unbiased metatranscriptomic next-generation RNA se...

Abstract Background With the tremendous rise in COVID-19 infection and shortage of real-time reverse transcription-polymerase chain reaction (RT-PCR) testing, we aimed to assess role CT detection correlation with patients’ management. A retrospective study was conducted on 600 patients who presented symptoms suspicious for between March end June 2020. The current followed RSNA recommendations r...

The coronavirus, which appeared in Wuhan city of China and named COVID-19 , spread rapidly caused the death many people. Early diagnosis is very important to prevent or slow spread. first preferred method by clinicians real-time reverse transcription-polymerase chain reaction (RT-PCR). However, expected accuracy values cannot be obtained patients incubation period. Therefore, common lung devast...

RAF1 (c-raf) has been known as an important tumor-promoter in many cancers. However, the regulatory mechanisms affecting expressions are rarely reported. This study aimed to predict candidate miRNAs of gene and verify their functions progression lung cancer. The expression miR-643 was detected by reverse transcription polymerase chain reaction (RT-PCR). A dual-luciferase report system used rela...

Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by ...

A variety of factors that influence real-time reverse transcriptase (RT)-PCR results were examined. Realtime reaction volumes can be decreased from the manufacturers suggested volumes (50μl) to as little as 5μl. Errors introduced through volume handlings can be significantly mitigated through the use of electronic pipettes. Random hexamers are compared against the use of a specific reverse tran...

Lumpfish are used routinely as cleaner fish to delouse farmed Atlantic salmon ( Salmo salar ) and often translocated globally. In September 2021, an aquaculture production business in Dorset, England experienced considerable mortalities consignments of lumpfish Cyclopterus lumpus imported from Norway. Following suspicion infection with virus (CLuV) by a veterinarian, commercial testing provided...

The reverse transcription polymerase chain reaction (RT-PCR), reverse passive haemagglutination assay (RPHA) and agar gel precipitation test (AGPT) were compared for the detection of infectious bursal disease virus (IBDV) in various organs of experimentally infected broilers. RT-PCR and RPHA required a time of 8 and 1 hour, respectively, whereas AGPT showed precipitation reaction at least after...