The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...

Liquid chromatography has been used as a means of egg protein analysis or as a method for the purification of egg proteins. Several chromatographic methods, including gel permeation, ion-exchange, reversed-phase, hydrophobic interaction, and immobilized-ligand-affinity chromatography, have been carried out for the separation or the purification of egg yolk or egg white proteins. Ion-exchange ch...

Comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infi nity 2D-LC Solution with DAD and MS detection is applied for the analysis of citrus oils. The developed method uses normal phase liquid chromatography in the fi rst dimension and reversed-phase liquid chromatography in the second dimension. This combination provides good orthogonality and is valuable for pro...

We compare measurement of the tricyclic antidepressants desipramine, imipramine, nortryptaline, and amitryptaline by reversed-phase "high-performance" liquid chromatography and by gas chromatography/mass fragmentography. The liquid-chromatographic procedure does not require ion-pairing reagents, and involves detection at 215 nm. The within-day and between-day CVs for this method were less than ...

Reversed-phase liquid chromatography (RP-LC) is a primary method chosen for protein characterization via peptide mapping. Peptide mapping applications require efficient columns to resolve complex peptide mixtures into unique peptides. Modified peptides, such as oxidized or deamidated ones, can also be separated from the unmodified peptides.1 UltraPerformance Liquid Chromatography® (UPLC®) techn...

Proteomics is the study of all or part of the protein complement of genes in an organism, often involving the analysis of complex protein/peptide samples. Such complex samples are beyond the separation capacity of 1-D separation techniques. This review describes several multidimensional separations for proteins and peptides. First, several variants of 2-D liquid chromatography (2DLC) are review...

Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethy...

−In reversed-phase liquid chromatography (RP-LC), the separation of ionized compounds at neutral pH of the mobile phase is ineffective because these compounds easily become ionized and too hydrophilic to adsorb on the hydrophobic C18 stationary phase. The common method to separate ionized compounds in RP-LC is the usage of the ion pairing agent and the control of pH in the mobile phase. In this...