Mucor miehei transformants resistant to geneticin have been obtained by treatment of protoplasts with different plasmids and by Agrobacterium-mediated DNA transfer. All transformants exhibited a very unstable phenotype whose maintenance required continuous selective pressure. Molecular analysis of transformants showed that the plasmid DNA was extensively modified and maintained in very low amou...

It has been shown that structured lipids, formed in the process of enzymatic modification natural hard fat with walnut oil, are capable stabilizing emulsion systems without need to add additional emulsifiers. This is especially true for emulsions containing during when amount added water reaction catalyst was range 12–16 wt%. Physicochemical evaluations, i.e., average particle size, its growth,...

Problems derived from obesity and overweight have recently promoted the development of fat substitutes and other low-calorie foods. On the one hand, fats with short- and medium-chain fatty acids are a source of quick energy, easily hydrolyzable and hardly stored as fat. Furthermore, 1,3-diacylglycerols are not hydrolyzed to 2-monoacylglycerols in the gastrointestinal tract, reducing the formati...

Short-chain triacylglycerol, tributyrin (glyceryl-sn-1,2,3-tri-butyric acids), was interesterified with palm oil to produce mixtures of structured triacylglycerol (SL-TAG) species as a low-calorie lipid. Lipozyme RM IM from Rhizomucor miehei, known as a 1,3regioselective immobilized lipase, was used as a catalyst. During 24 h reaction, the reaction mixture was analyzed with HypersilBDSCPS high ...