نتایج جستجو برای: safranin
تعداد نتایج: 522 فیلتر نتایج به سال:
Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage da...
Purpose: The objects of this study were to compare biological properties of a native articular cartilage extracellular matrix (ACECM)-derived oriented scaffold and the Chondro-Gide bilayered scaffold. Methods: The ACECM oriented scaffold (No. 4131) and the Chondro-Gide scaffold (No. 4132) compared form (I) The physical properties (II) FDA/Pl staining, and frozen sections were stained with tolui...
PURPOSE To evaluate multiple MRI parameters in a surgical model of osteochondrosis (OC) in goats. METHODS Focal ischemic lesions of two different sizes were induced in the epiphyseal cartilage of the medial femoral condyles of goats at 4 days of age by surgical transection of cartilage canal blood vessels. Goats were euthanized and specimens harvested 3, 4, 5, 6, 9 and 10 weeks post-op. Ex vi...
Kidney proximal tubules develop a severe but highly reversible energetic deficit due to nonesterified fatty acid (NEFA)-induced dissipation of mitochondrial membrane potential (DeltaPsi(m)) during reoxygenation after severe hypoxia. To assess the mechanism for this behavior, we have compared the efficacies of different NEFA for inducing mitochondrial deenergization in permeabilized tubules meas...
The effect and related mechanisms of miR-127-5p on the cartilage differentiation of rat bone marrow mesenchymal stem cells (BMSCs) was investigated. Rat BMSCs were generated and transfected with miR-127-5p, RT-PCR and Safranin O staining were used to detect the effect of miR-127-5p on the cartilage differentiation of rat BMSCs. Western blot analysis was used to detect the related mechanisms of ...
Bone marrow-stimulating techniques such as microfracture and subchondral drilling are valuable treatments for full-thickness cartilage defects. However, marrow stimulation-derived reparative tissues are not histologically well-documented in human osteoarthritis. We retrospectively investigated cartilage repairs after marrow stimulation for the treatment of large cartilage defects in osteoarthri...
Many staining protocols are widely applied in botanical microtechniques and serve specific histological purposes. In particular, some dyes used simultaneously to receive contrasting colorations of different chemical structures, e.g., lignin cellulose. One the most popular differential is based on Safranin O / Astra Blue combination. a water-soluble basic dye that stains red. also but having an ...
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