Activation of neutrophils by phagocytosis or certain soluble stimuli results in a burst of oxidative metabolism and in degranulation. Fluoride is known to trigger the metabolic burst. but degranulation has not been reported. lodination of protein by F -activated human PMN was demonstrated by measuring TCA-precipitable 1251. lodination was dependent on H20,. since it was inhibited by catalase. e...

An iron(III)-catalyzed method for the rapid and highly regioselective iodination of arenes has been developed. Use of the powerful Lewis acid, iron(III) triflimide, generated in situ from iron(III) chloride and a readily available triflimide-based ionic liquid allowed activation of N-iodosuccinimide (NIS) and efficient iodination under mild conditions of a wide range of substrates including bio...

OBJECTIVE AND METHODS Previous studies have pointed out that Ribeirão Preto, São Paulo, is an area of borderline iodine deficiency, later becoming a region of excessive iodination. In a reevaluation of the iodine status of the city in 2007-2008, 300 schoolchildren aged 8 to 10 years were evaluated, from 3 schools of 3 different socioeconomic levels who were submitted to the determination of iod...

A discontinuous gradient of Percoll was used to remove RBCs from polymorphonuclear leucocyte (PMN) preparations. The resulting red blood cell-free preparation was used to investigate the effect of ammonium chloride on the iodination response of human PMN. Treatment of PMN with ammonium chloride for five minutes at room temperature resulted in a statistically significant increase in the iodinati...

1. A method is described for the trace iodination of immunoglobulins and other serum proteins by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. gammaG immunoglobulin that had been labelled to a specific radioactivity of 5muc/mug. by use of carrier-free [(125)I]iodide gave no evidence of denaturation when analysed by electrophoresis and density-gradient ultracentrifugat...

A double labeling procedure was employed to determine whether the tyrosine residues in thyroglobulin that are iodinated first are also those that are most readily converted to thyroxine. Goiter thyroglobulin containing less than 1 atom of iodine per molecule was iodinated first with 9 at relatively low levels of iodination (5 to 20 atoms of iodine per molecule) and afterward with lzjI at higher...

Introduction The conversion of tyrosyl residues into iodo-derivatives can be brought about under different ex­ perimental conditions [1 -3 ] and is essentially an electrophilic substitution by iodine at the phenolic ring of tyrosine. This reaction has been performed for different purposes in protein chemistry, e.g. the iodination of tyrosine has been extensively used in the preparation of label...

Current methods for assessment of iodine intake in a population comprise measurements of urinary iodine concentration (UIC), thyroid volume by ultrasound (US-Tvol), and newborn TSH. Serum or dried blood spot thyroglobulin (DBS-Tg) is a new promising functional iodine status biomarker in children. In 1996, a new act on universal salt iodination was introduced in Croatia with 25 mg of potassium i...