A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa. The enzyme showed activity towards several substrates, the most sensitive of which was 2,2'-Azinobis-(3-ethylbenzthiazoli...

Aminopeptidase C was purified from fresh chicken skeletal muscle by arnmonium sulfate fract;onation,andbysuccessivechromatographyonDEAE-cellulose,UltrogelAcA34,DEAE-cellulose again, and an alanine AH-Sepharose 4B ftMnity column twice. The purificd enzyme migrated as a s;ngle band by SDS-PAGE. Am;nopeptidase C was purified about 300-fo1cl over the crllde extraet with a },ield of O.6%. The molecu...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened for the production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneity on a single step by affinity chromatography using insoluble corn starch. The molecular weight of Amy RM44 was estimated to be 66 kDa by SDS-PAGE and zymographic analysis. Nine fold purification was achieved with the specifi...

The prophenoloxidase activating system (proPO-AS) in fresh water crab, Barytelphusa cunicularis is localized in the hemocytes. Using anticoagulant (EDTA-Citrate buffer) it was possible to isolate intact hemocytes without causing extensive degranulation & subsequent clotting. Activation and conversion of prophenoloxidase to phenoloxidase(PO) was carried out by serine proteinase. PO was found to ...

Protease enzymes are that able to hydrolyze peptide bonds in proteins into simple compounds. The protease enzyme used was derived from fermented sea cucumber digestive organs (HSFI-8). HSFI-8 uses meat tenderizer as well an anticoagulant agent. samples were beef, chicken and tuna whose protein profile analyzed using SDS-PAGE. Three types of soaked at a concentration 30% v/v for 3 h. In addition...

Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, serrapeptase and in vitro thrombolytic effects were investigated. The results showed that was 1295 U/mg, specific 3867 U/mg protein when toward casein 2800 U/mg. optimum temperature pH 37-40°C 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ Fe2...

In this study, some biological activities of Convallaria majalis L. (Asparagaceae), which grows naturally in Muğla and its surroundings attracts attention with pleasant smell, were determined, the isolation drug active substances industrial usability investigated. For purpose, protease enzyme that catalyzes hydrolysis proteins, is one most important groups both biochemical applications, into pe...

Alkaline transfer to nylon membranes is a widely used modification of the Southern blot procedure (1). However, several reports describe decreased hybridization efficiency with alkaline transfer (2,3,4). Shorter exposure time to alkali, or lower alkali concentration have been suggested to increase hybridization efficiency. We report an example of altered DNA banding following alkali transfer co...

Bacillus sp. ASX42 isolated from soil samples of Lake Van shores, Turkey, and the strain producing cellulose-free xylanase enzyme with an optimum pH 9.0, at 60ºC. The molecular weight was estimated as 66 kDa on SDS PAGE analysis. Thermal stability detected average 68.7% 4-60ºC 60% 65-95ºC while observed about 90% between 3.5-13.0 for 15 min. As HgCl2 presented a strong inhibitor activity, Cobal...

Thermophilic Bacillus sp. GUS1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. The enzymes were stable in the alkaline pH range (8.0-12.0), with the optimum temperature and pH range of the proteases being 70ºC and 6.0-12.0, respectively. All th...