The binding of bilirubin was analysed in the serum of a patient with unusual myeloma. This patient presented an acquired Von Willebrand's disease due to the presence of a ternary complex formed by one molecule of myeloma immunoglobulin G and two molecules of albumin (Harlé et al. La Presse Médicale 19, 1661-1664 (1990)). The binding characteristics of bilirubin--number of sites (n) and associat...

Difference spectroscopy is used to monitor bilirubin-protein interactions, to assess the residual binding capacity of proteins for bilirubin. A change in the difference spectra monitored at 482 nm is directly proportional to bound bilirubin up to a molar ratio of bilirubin to albumin of approximately 1; increasing bilirubin beyond the 1:1 molar ratio does not further change the difference spect...

Administration of Sn-protoporphyrin to Gunn rats that are characterized by a genetically determined absence of UDP-glucuronyl transferase activity for bilirubin, 24-30 h after birth, prevented the marked increase in serum bilirubin concentration that occurs in these animals in the postnatal period. A second administration of Sn-protoporphyrin at day 6 maintained serum bilirubin levels in the ne...

BACKGROUND Gilbert's syndrome (GS) is the most common inherited disorder of bilirubin metabolism. Recent data show that the rifampin test can be used as a diagnostic test but there is controversy about its effect on bilirubin level in normal individuals. We studied the effect of administration of rifampin on serum bilirubin level in patients with GS and in healthy individuals. METHODS Serum t...

The transcutaneous bilirubinometer was evaluated in 60 term and 10 preterm infants. A significant correlation was found between the transcutaneous index and the total serum bilirubin concentration for both term and preterm infants. The reliability of the transcutaneous bilirubinometer as a screening method was confirmed, and index criteria for serum bilirubin analysis have been suggested for te...

This chapter will focus on techniques that have been developed to measure transcutaneous bilirubin (TcB). The first electronic TcB device proved to be useful when used as a screening method for identifying newborns who needed a serum bilirubin determination. Newer TcB devices can be used not only as screening tools but also as reliable substitutes for serum bilirubin measurements. The Chromatic...

Objectives: Jaundice is a main problem in the early neonatal period and it is suggested that there is a positive correlation between plasma ionized Mg levels and severity of hyperbilirubinemia in newborn. The aim of this study is to explore the association and to evaluate and interpret its clinical significance as well as to observe the effect of phototherapy on serum magnesium and bilirubin le...

Site-directed mutagenesis of human serum albumin was used to study the role of various amino acid residues in bilirubin binding. A comparison of thermodynamic, proteolytic, and x-ray crystallographic data from previous studies allowed a small number of amino acid residues in subdomain 2A to be selected as targets for substitution. The following recombinant human serum albumin species were synth...

Aim: To compare neonatal serum bilirubin and certain haematological factors in Normal, induced and Caesarean section. Materials and Methods: 90 neonates were divided into three groups according to the mode of delivery (normal, oxytocin induced vaginal delivery & caesarean) and neonatal serum bilirubin levels were estimated and compared with each group. Result: Out of the 90 subjects, it was fou...