Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Seru...

The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mM Ca2+, epidermal growth factor, transferrin, insulin, and Na2SeO3, were fibroblast free, exhibited saturation densities similar to those of cells grown in se...

Freshly explanted lymphocytes and hemopoietic cells can grow and differentiate to terminally functioning cells in culture. Such cultures provide an opportunity to study, in cells not previously subjected to in vitro selection, such phenomena as induction of growth in physiologically resting cells, immune recognition, and the function of specific polypeptide factors and cell surface receptors. T...

In general, it is still impossible, except in a few instances (7, 9), to cult ivate an imal and h u m a n cells successfully in serum-free medium. W h e n cul t ivated under such conditions, the cells proliferate for only a l imited period of t ime and then stop. I t seems reasonable to assume tha t differences in the consti tuents of the med ium which have such a marked influence on pat terns ...

Abstract: Although serum is a main source of essential nutrients and growth factors, its use creates highly complex and poorly defined media. The use of serum-free media is more efficient than the use of media containing serum especially when the purification and characterization of antibodies are considered. In this study, we developed a serum-free medium system for cultivation of hybrid cells...

The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mw Ca2+, epidermal growth factor, transferrin, insulin, and Na2SeO3, were fibroblast free, exhibited saturation densities similar to those of cells grown in se...