Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1 VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive r...

of the Joint International Tropical Medicine Meeting (JITMM). Bangkok, Thailand. 29 November-1 December 2004:106. EVALUATION OF A SINGLE-DILUTION PLAQUE REDUCTION NEUTRALIZATION TEST (PRNT) AS A DIAGNOSTIC TOOL FOR DETECTING INTERCURRENT DENGUE VIRUS (DENV) INFECTIONS Thomas SJ, Nisalak A, Halstead S, Endy T, Lutthiwongsakorn N, Poolpanichupatam Y, Sawatwong P, Changnak S and Mammen MP Jr Studi...

The prevalence of BHV-1 and BLV antibodies was studied in 313 serum samples collected from 5 dairy cattle herds. Serum samples were collected from both apparently healthy animals and animals with respiratory symptoms. The samples were tested for the presence of antibodies against BHV-1 and BLV using virus neutralization and agar gel immunodiffusion test, respectively. The results indicated that...

A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relation...

Specimens were obtained from the 3 Malagasy fruit bats, Pteropus rufus, Eidolon dupreanum, and Rousettus madagascariensis. Antibodies against Nipah, Hendra, and Tioman viruses were detected by immunoassay in 23 and by serum neutralization tests in 3 of 427 serum samples, which suggests that related viruses have circulated in Madagascar.

Equine antisera to human adenovirus types 1 to 33 were prepared and evaluated by hemagglutination-inhibition and serum neutralization tests. Detailed data on the potency and purity of the immunizing antigens were tabulated as one means of evaluating the antisera. Most of the 52 hemagglutination-inhibition and 25 serum neutralization major or minor heterotypic responses among the equine antisera...

EU Regulation 998/2003 requires the serological testing of rabies-vaccinated dogs and cats in approved laboratories using serum neutralization tests prior to movement of pet animals between certain EU member states and before pet animals are imported from unlisted third countries. Serum neutralisation tests are also used for measuring the efficacy of oral rabies vaccination programmes conducted...

In the USA, the potency of commercially prepared equine tetanus antitoxin is determined by the method outlined in the Code of Federal Regulations, Title 9, Part 113.451. In the current test, commercial equine tetanus antitoxin is tested by a toxin neutralization test in guinea pigs. The in vivo test measures antitoxin content through effectiveness of protection of guinea pigs injected with dilu...

BACKGROUND & OBJECTIVES The reports from the countries where mumps vaccine is given as routine immunization suggest differences in mumps virus neutralizing antibody titres when tested with vaccine and wild type viruses. Such reports are unavailable from countries like India where mumps vaccine is not included in routine immunization. We, therefore, undertook this study to understand the cross-n...