Proteins that interact coevolve their structures. When mutation disrupts the interaction, compensation by the partner occurs to restore interaction otherwise counterselection occurs. We show in this study how a destabilizing mutation in one protein is compensated by a stabilizing mutation in its protein partner and their coevolving path. The pathway in this case and likely a general principle o...

inhibiting subtilisin BPN'. This paper describes purification and characterization of a subtilisin inhibitor (FMSI) from foxtail millet grain. Protein was measured by the method of Lowry et al.2) using bovine serum albumin as a standard. Protein concentrations of enzyme solutions were measured spectrophotometrically with extinction coefficients as described previously.3) The concentration of St...

Broomcorn and foxtail millet are the only major domesticated plants indigenous to prehistoric Eurasia rely on C4photosynthetic pathway. Here we study impact of animal manure (AM) broomcorn (Panicum miliaceum) (Setaria italica), grown in Askov Long-Term Experiment, using unmanured soil dressed with phosphorus plus potassium (PK) as reference treatments. Animal had a marked effect yields δ15N val...