نتایج جستجو برای: shotgun assembly sequences
تعداد نتایج: 336465 فیلتر نتایج به سال:
The chicken genome has a haploid content of 1.2 x 109 base pairs (bp) of DNA; approximately 40% that of either mouse or human. The strategy that will be employed to sequence this genome is to assemble 6-fold whole-genome shotgun coverage of the genome and to order and orient the resulting sequence scaffolds by alignment to endsequences of BACs in a comprehensive contig map. This is in contrast ...
It seems appropriate that the Cold Spring Harbor Genome Sequencing and Biology Meeting, which witnessed the creation of the Human Genome Organization (HUGO) in 1988, should this year present three major advances in genomic science: the completion of the ®nished sequence of Drosophila melanogaster; the announcement that 85% of the genome of Homo sapiens is now in draft sequence; and the complete...
In spite of the growth of molecular ecology, systematics and next-generation sequencing, the discovery and analysis of diversity is not currently integrated with building the tree-of-life. Tropical arthropod ecologists are well placed to accelerate this process if all specimens obtained through mass-trapping, many of which will be new species, could be incorporated routinely into phylogeny reco...
We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs by virtue of end-sequencing 2-kbp, 10-kbp, and 50-kbp inserts from shotgun clone libraries. The quality-trimmed reads covered the genome 5.3 times, and the inserts from which pairs of reads were...
ABSTRACT Sequence assembly is an essential requirement for determining the complete sequence of long DNA. However, sequence assembly programs often generate misassembled contigs by either joining di erent repeat copies, resulting in joining non contiguous DNA regions (inverted or swapped) or by including many fragments from di erent repeat copies resulting in errors in the consensus sequence (n...
33 T he prevailing method of determining the sequence of a long DNA segment is the shotgun sequencing approach, in which a random sampling of short fragment sequences is acquired and then assembled by a computer program to infer the sampled segment's sequence. In the early 1980s, such segments were typically on the order of 5,000 to 10,000 base pairs (5 to 10 kbp). By 1990, this method was sequ...
RATIONALE Lung infections caused by opportunistic or virulent pathogens are a principal cause of morbidity and mortality in HIV infection. It is unknown whether HIV infection leads to changes in basal lung microflora, which may contribute to chronic pulmonary complications that increasingly are being recognized in individuals infected with HIV. OBJECTIVES To determine whether the immunodefici...
'Mitochondrial metagenomics' (MMG) is a methodology for shotgun sequencing of total DNA from specimen mixtures and subsequent bioinformatic extraction of mitochondrial sequences. The approach can be applied to phylogenetic analysis of taxonomically selected taxa, as an economical alternative to mitogenome sequencing from individual species, or to environmental samples of mixed specimens, such a...
Analysis of a complex protein mixture is one of the main topics in proteomics. While the traditional 2D-PAGE method separates proteins first and then identifies each spot, an alternative method was proposed which cleavages the whole protein mixture with an enzyme like trypsin, separates the resulted peptide fragments with multi-dimensional LC, identifies each fragment with mass spectrometry, an...
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