Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response an...

The small asaccharolytic, nonpigmenting gram-negative rods of the human oral cavity are difficult to differentiate from each other. Protein profiles of sonicated cells of Wolinella species, Campylobacter concisus, Bacteroides gracilis, and Eikenella corrodens were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized with a silver stain. The gels were scanned with...

The rodent endometrium undergoes remarkable modifications during pregnancy, resulting from a redifferentiation of its fibroblasts. During this modification (decidualization), the fibroblasts transform into large, polyhedral cells that establish intercellular junctions. Decidualization proceeds from the subepithelial stroma towards the deep stroma situated next to the myometrium and creates regi...

Cu-Ag particles with homogeneous cover-silver layer were synthesized by electroless plating of copper particles with silver sulfate using eco-friendly sodium citrate (SC) as reducing agent, dispersant and chelating agent in an aqueous system. The influences of sodium citrate/Cu and sodium citrate/Ag ratios on Ag coatings of Cu powders were investigated. Ag formed a dense coating on the surface ...

A modified Neuhoff's colloidal Coomassie Blue G-250 stain is reported, dubbed "blue silver" on account of its considerably higher sensitivity, approaching the one of conventional silver staining. The main modifications, as compared to Neuhoff's protocol, were: a 20% increment in dye concentration (from 0.1% up to 0.12%) and a much higher level of phosphoric acid in the recipe (from 2% up to 10%...

In 1932 (1) the author published a method for determining methionine in proteins based on the recovery of methyl iodide. Alcoholic silver nitrate was used as absorbant and the excess of silver nitrate was titrated with thiocyanate after filtering off the silver iodide. This method was quite satisfactory and has been used recently by Barritt (2) for the determination of methionine in wool. Certa...

Bioleaching processes for extraction of Cu and Mo from molybdenite cons. are more environmentally friendly and consume less energy than conventional technologies, yet less economically efficient. One necessary step towards arriving at a cost-effective bioleaching process is using appropriate methodology to optimize pertinent factors in such processes. To this end, the present study employed Res...

La0.8Sr0.2MnO3 (LSM) catalyst is prepared via a sol-gel method and modified typical silver mirror reaction. Silver ammonia solution reduced in polyvinylpyrrolidone (PVP)-containing to obtain nanoparticles sodium dodecyl sulfate (SDS) added as surfactant. The microstructure morphology of the LSM-Ag composite powder are characterized. According results, Ag particles precipitate on LSM surface ele...

Chondroitin sulfate is known to be present in normal and leukemic myeloid cells; however, its definitive subcellular location and association with other glycosaminoglycans (GAGs) has not been demonstrated. We have studied the type and distribution of GAGs in neutrophil granule subpopulations of normal and leukemic myeloid cells using ultrastructural, cytochemical, immunologic, and biochemical m...

The lipopolysaccharides (LPSs) of five isolates of Pasteurella multocida from rabbits were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblots, and enzyme-linked immunosorbent assay. Silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of purified unaggregated LPSs resembled those of semirough strains of gram-negative enterobacter...