Background: The aim of this study was to develop methods for cryopreservation and long-term maintenance of putative bovine embryonic stem cells (ESCs). Materials and Methods: Putative bovine ESC (bESC) lines (n=3) isolated in conventional medium were used to compare slow-freezing and vitrification. Results: After warming, vitrified cells (96.9%) demonstrated significantly (p<0.05) better surviv...

Cracks can form during the freeze-drying of freeze cast ceramic suspensions while attempting to produce dense ceramics. The contain alumina particles dispersed in cyclohexane. rate drying is controlled by pressure and temperature (slow at atmospheric −15°C fast under vacuum slowly increases from −80°C room temperature). X-Ray micro-computed tomography was used characterize internal crack format...

BACKGROUND Ovarian tissue cryopreservation may be a potential method of preserving fertility in women who have experienced gonadotoxic treatments. To improve the efficiency of existing cryopreservation, we developed a practical and convenient vitrification method named needle immersed vitrification (NIV), which required a less concentrated and minimum volume of vitrification solution. METHODS...

A problem of the noise-induced generation and shifts phantom attractors in nonlinear dynamical systems is considered. On basis model describing interaction climate vegetation we study probabilistic mechanisms systematic global temperature both upward (“warming”) downward (“freezing”). These are associated with changes area Earth covered by vegetation. The mathematical these phenomena performed ...

OBJECTIVE To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts. DESIGN Prospective in vitro study. SETTING Tertiary care academic hospital. INTERVENTION(S) 1) Twenty-two expanded blastocysts (EB) and 17 nonexpanded blastocysts (NEB) vitrified in cryotips; 2) 15 EB and 16 NEB by slow freezing using p...

This study aims at providing new insight on protein denaturation in freezing-thawing. Freezing-thawing minced pork reduced water-holding of myofibrils and increased surface hydrophobicity. One additional freezing-thawing cycle slow freezing rate caused appearance a 160 kDa myosin-4 fragment SDS-PAGE, further decreased Fresh was exposed to either high salt (2 M KCl) only or with lower pH mimic c...

To examine effects of varied freezing conditions on the development of spatial heterogeneity in the frozen protein solution, macroscopic freeze concentration and micro-segregation of bovine serum albumin (BSA) were investigated in a temperature-controlled 200-ml freeze container. Freezing to -40 °C promoted formation of protein concentration gradients (69-114 μg ml-1) in frozen samples taken fr...

Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (...

Ovarian tissue cryopreservation is the key step towards the establishment of an ovarian tissue bank or the preservation of ovarian tissue for patients scheduled for gonadotoxic cancer therapies, aiming for fertility restoration later on. Conventional cryopreservation, or slow freezing, has been the mainstay of ovarian tissue cryopreservation. Vitrification has recently emerged as a new trend fo...