Although direct sequencing is the gold standard for KRAS mutation detection in routine diagnostics, it remains laborious, time consuming, and not very sensitive. Our objective was to evaluate SNaPshot and the KRAS StripAssay as alternatives to sequencing for KRAS mutation detection in daily practice. KRAS exon 2-specific PCR followed by sequencing or by a SNaPshot reaction was performed. For th...

background: cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. this study aimed to compare the perfor-mance of several molecular methods for identification of cryptosporidium species, and to detect genetic variation among each of these species isolated from iran, ma-lawi, nigeria, vietnam and the united kingdom. methods: the oocysts dna...

While snapshots have been commonly used in data storages for backup and data protections, little is known in the open literature how such snapshots impact application performance. This paper presents an implementation and performance evaluation of two snapshot techniques: copy-on-write snapshot and redirect-on-write snapshot. Our implementation is carried out at block level on a standard iSCSI ...

Applications need to analyze the past state of their data to provide auditing and other forms of fact checking. Retrospective snapshot systems that support computations over data store snapshots, allow applications using simple data stores like Berkeley DB or SQLite, to provide past state analysis in a convenient way. Current snapshot systems however, offer no satisfactory support for computati...

Background Previous studies have indicated that nitric oxide synthase 2 ( NOS2 ) genetic variations are involved in delayed fracture healing and non-union. Whether these variants associate with the development of osteomyelitis (OM) remains unclear. Here, we analyzed potential relationships between risk developing post-traumatic OM (PTOM) a Chinese Han population. Methods Altogether 704 particip...

Due to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required. Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked. We examined KRAS mutations in exon 2 codons 12 and 13...

A mobile computing system consists of mobile and stationary nodes, connected to each other by a communication network. The presence of mobile nodes in the system places constraints on the permissible energy consumption and available communication bandwidth. To minimize the lost computation during recovery from node failures, periodic collection of a consistent snapshot of the system (checkpoint...

An atomic snapshot object is an object that can be concurrently accessed by asynchronous processes prone to crash. It is made of m components (base atomic registers) and is defined by two operations: an update operation that allows a process to atomically assign a new value to a component and a snapshot operation that atomically reads and returns the values of all the components. To cope with t...