We used small-angle X-ray solution scattering (SAXS) technique to investigate the nucleotide-mediated conformational changes of the head domains [subfragment 1 (S1)] of myosin V and VI processive motors that govern their directional preference for motility on actin. Recombinant myosin V-S1 with two IQ motifs (MV-S1IQ2) and myosin VI-S1 (MVI-S1) were engineered from Sf9 cells using a baculovirus...

while (1) { with (s1) /* send r to 2d shape */ where (s1mask) pcoord(0)]]0]conn = r; with (s2) where (s2mask) { conn = copy_spread(&conn, 1, 0); /* spread */ conn &= e; /* combine */ reduce(&conn, conn, 0, /* reduce */ CMC_combiner_logior, 0); where (pcoord(0)==0) pcoord(1)]temp = conn; /* return result */ } with (s1) { /* stop if r hasn't changed */ if (&=(temp==r)) break; r = temp; } } CM_tim...

Several molecules absorb light energy which they emit after a time difference (lifetime) as radiation energy. Molecules remain at a low energy level or the ground electronic singlet state (So) or the lowest vibrational level at room temperature (Noomnarm and Clegg, 2009). On absorption of a photon, the molecule is excited from So to the first electronic excited singlet state S1 within < 10-15 s...

Penelitian bertujuan untuk menggambarkan kebutuhan akan metode pembelajaran yang tepat pada perkuliahan statistik penelitian pendidikan dimasa covid-19. Jenis digunakan adalah deskriptif kuantitatif. Sampel berjumlah 59 mahasiswa terdiri dari 20 S2 IPA dan 39 S1 Fisika disalah satu Universitas Negeri Di Kota Bengkulu. Instrumen berupa angket respons 8 item pertanyaan. Pengumpulan data dilakukan...

Here we briefly review our information theoretic approach to revealing community structure in weighted and directed networks [1] and present a new fast stochastic and recursive search algorithm to minimize the map equation — the objective function of our method. We have developed this algorithm to be able to accurately partition the large number of bootstrap networks [2]. The search algorithm c...

The double exponential model t t M T Be Ae vl μ μ − − + = was fitted to log10(vl) data from day 0 to day 28 post ART. When vl was undetectable it was replaced with the limit of detection (80 copies per ml), indicated by the dashed line in Figure S1 and S2. Fits were performed by nonlinear least squares regression, confidence intervals were calculated by bootstrapping the cases and trimming the ...

There are several downstream analysis goals for which RNA-seq is well suited. Main categories of these are described briefly below with reference to supporting materials. Refer to S2 Table for specific tools relevant to many of these areas. For each application, a basic data recommendation is provided. It is important to remember that these are simply examples. In addition to the varying demand...