The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72-96 hr and thereby increase the yield significantly. Cell c...

References 1. Khayam-Bashi, H., and Sims, C., Effect of sodium aside on the quantitation of chemical constituents of serum. Am. ,J. Clin. Pat hol. 69, 405 (1978). 2. Khayam-Bashi, H., Sims, C., and Parekh, A. C., Interference of sodium azide with the quantitation of serum cholesterol: A comparative study. Clin. Chim. Acta 66, 69 (1976). 3. Queen, A., and Frings, C. S., Effect of sodium azide on...

methanesulfonic acid (msa) was found to be an efficient catalyst for the synthesis of 1-substituted-1h-1,2,3,4-tetrazoles. a series of 1-substituted tetrazole compounds were synthesized from the reaction of various primary amines, sodium azide and triethyl orthoformate in the presence of catalytic amounts of msa at room temperature. in this protocol, some of the tetrazole derivatives were synth...

A recent Letter by Eckfeldt et al. (1) recalled some of our unpublished findings of a few years ago, showing the effect of sodium azide on continuous-flow (SMA 12/60) measurements (Table 1). The azide interference with total bilirubin determinations they reported can be also be seen in our results, although not conspicuously. As can be seen in Table 1, measurements other than bili-. rubin may b...

The effects of several inhibitors on the rates of photosynthesis and of respiration by Chlorella pyrenoidosa have been studied. The inhibitors used in this study include potassium cyanide, hydroxylamine hydrogen chloride, dinitrophenol,and sodium azide. Each inhibitor seems to have its own characteristic action in photosynthesis and in respiration. With the exception of hydroxylamine, all the i...

Some aryl and heteroaryl azido compounds were synthesized from the reaction of corresponding fluoro and chloro compounds with sodium azide in DMF as solvent and under various reaction conditions especially ultrasonic irradiation. Reaction of aromatic and heteroaromatic azido compound with thioacetic acid in the presence of sodium hydrogen carbonate and in methanol as solvent led to the differen...

Five laboratory technicians accidentally ingested sodium azide, in quantities varying from 20 to 80 mg, in tea made from distilled water kept in a laboratory. Four had short-lived symptoms of dizziness, pounding heart, and faintness, while another developed a picture of myocardial ischaemia. He had further episodes of chest pains necessitating admission to hospital. These later symptoms were th...