Expression of myelin basic protein (MBP) in mice is regulated in a cell- and stage-specific manner during brain development. The MBP control region contains multiple cis-acting elements, shown by in vivo and in vitro assays, which are responsible for its unique pattern of transcription. Using synthetic DNA fragments spanning the MBP control region, we have analyzed nuclear proteins obtained fro...

Here we report the isolation and initial biochemical characterization of a 120-kD peanut agglutinin-binding glycoprotein from the adult human central nervous system (CNS), which is anchored to membranes through a phosphatidylinositol linkage. Myelin incubated with phosphatidylinositol-specific phospholipase C released the protein as a soluble polypeptide of 105 kD, which was isolated with peanu...

Three cannulated Tswana steers were used to investigate the rumen degradability of mopane worm (Imbrasia belina) by measuring the amount of dry matter (DM) and crude protein (CP) disappearing at incubation periods up to 72 h. The effective degradability (ED) of DM and CP in the rumen was calculated at outflow rates of 0.03 / h (ED0.03) and 0.05 / h(ED0.05). Rumen degradable CP (RDP) was estimat...

Selection of a system for successful recombinant protein production is important. The aim of this study wasto produce high levels of human interleukin-2 (hIL-2) in soluble form. To this end, the pET32a vector inEscherichia coli BL21 (DE3) was used as an expression system, since it was previously used for the productionof mouse IL-2 in soluble form. The results indicated that c...

A sweet water-soluble protein that reminds stable over wide ranges of temperature and pH, Brazzein has various applications. Its tastes like cane sugar but have no calories. However, the extraction of brazzein from its natural source is expensive and not applicable. In this study we used recombinant DNA technology to provide an alternative option for cheaper mass production of brazzein. A brazz...

AIM Active vitamin D (1,25-dihydroxyvitamin D₃), PTH, fibroblast growth factor-23 (FGF-23) and Klotho protein are key regulators of phosphate metabolism. Hyperphosphatemia and increased FGF-23 level in patients with end-stage renal disease are associated with increased morbidity and mortality. The relationships among key regulators of phosphate metabolism are still being investigated. FGF-23, t...

intercropping is considered as a method for increasing yield per unit and stability. in order to evaluate the effects of phosphorous biofertilizer and chemical phosphorous on fodder quality of corn (zea mays l.) and grass pea (lathyrus sativa l.) intercropping, two field experiments were conducted as factorial based on randomized complete block design with four replications at two places simult...