The pathologic role of autoantibodies in many autoimmune diseases is widely accepted. An enzyme immunoassay was used for measurement of antibodies against disease-specific antigens and etiologic agents for cross-reactive antigens associated with them. This antibody assay was applied to a panel of antigens for the detection of different neuroautoimmune diseases that included multiple sclerosis, ...

Four commercially available Aspergillus fumigatus antigens were compared in an IgG enzyme linked immunosorbent assay by testing multiple sera from 19 patients who had received renal and hepatic allografts and who had histologically confirmed invasive aspergillosis. Raised antibody levels were found in 16 of the 19 patients. More antibody responses were detected by culture filtrate antigens, but...

strongyloidiasis, caused by a nematode parasite so-called strongyloides stercoralis is one of the major human intestinal nematode infections. considering that stool examination for strongyloides larvae is not a sensitive method and immunodiagnostic methods are more applicable for this purpose, so the present study was conducted to compare the somatic (s) and excretory - secretory (es) antigens ...

Antigens derived from somatic extracts of Bithynia funiculata, an intermediate snail host of O. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (MW). These antigens have been suggested to be of potential use for serodiagnosis. In this study, B. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (C1); 20,000 (C2); 30...

fasciolosis caused by fasciola hepatica and f.gigantica is one of the major public health problems in the world and in iran. considering that stool examination for fasciola eggs is not a sensitive method and only 25% of infected patients pass the eggs in the faeces , and immunodiagnosis methods are more applicable for this purpose, the present study was conducted to compare the somatic (s) and ...

Shands, J. W. (University of Florida, Gainesville). Localization of somatic antigen on gram-negative bacteria by electron microscopy. J. Bacteriol. 90:266-270. 1965.-Antisera specific for the somatic antigens of Salmonella typhimurium and Escherichia coli O113 were prepared, and globulins isolated from these antisera were labeled with ferritin. Micrographs of labeled, sectioned bacteria show th...

BACKGROUND There are no established methods for the preparation and standardization of Fusarium solani antigens. This lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests. OBJECTIVE To obtain an appropriate standardized method for the preparation of the different antigen types of F. solani. METHODS Production of fungal extracts, followed by biochem...