نتایج جستجو برای: spa typing

تعداد نتایج: 26930  

2017
Haishen Kong Fei Yu Weili Zhang Xuefen Li Hongxia Wang

Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to spa typing, SCCmec typing, multiple locus sequence typing (MLST), and PCR...

2015
Sophie Roussel Benjamin Felix Noémie Vingadassalon Joël Grout Jacques-Antoine Hennekinne Laurent Guillier Anne Brisabois Fréderic Auvray

Staphylococcal food poisoning outbreaks (SFPOs) are frequently reported in France. However, most of them remain unconfirmed, highlighting a need for a better characterization of isolated strains. Here we analyzed the genetic diversity of 112 Staphylococcus aureus strains isolated from 76 distinct SFPOs that occurred in France over the last 30 years. We used a recently developed multiple-locus v...

ژورنال: طب جنوب 2014
آموزنده نوباوه, علی‌رضا, غزنوی‌راد, احسان‌اله,

زمینه: در این تحقیق روش‌های مولکولی موجود جهت تیپ‌بندی اپیدمیولوژیک استاف اورئوس مقاوم به متی‌سیلین مانند روش الکتروفورز در ژل با میدان الکتریکی ضربان‌دار (PFGE)، تیپ‌بندی بر مبنای توالی‌یابی لوکوس‌های چندگانه (MLST)، تیپ‌بندی بر مبنای توالی‌یابی ژنی پروتئین A (Spa) Staphylococcus aureus protein A typing، تیپ‌بندی به‌روش واحد تکراری مستقیم (dru)Direct repeat unit typing و بررسی تنوع در کاست کرو...

2010
Claudio Zweifel

! Genotypisierung der 20 gefundenen MRSA-Stämme mittels Multilocus-Sequenz-Typisierung sowie Charakterisierung des Staphylokokken Proteins A (spa typing) und Typisierung des staphylococcal cassette chromosome mec (SCCmec).

Journal: :Collegium antropologicum 2016
Ana Budimir Brigita Tićac Tomislav Rukavina Maja Farkaš Smilja Kalenić

The aim of the study was to investigate the molecular epidemiology of community- associated MRSA in Primorsko-Goranska County of Croatia during a six-year period(2001-2007). In period from 2001 and 2007, 46 MRSA isolates were collected in Rijeka, strains were subjected to susceptibility testing according to CLSI guidelines, mecA gene detection and SCCmec typing as well as detection of PVL. Stra...

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital- and community-acquired infections. USA300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. Methods: Totally 171 isolates of S. aureus were collected fro...

2011
JJ Hirvonen T Pasanen S Mero S-S Kaukoranta M Vaara J Vuopio S Salmenlinna P Tissari

Methods The usefulness of spa-typing, pulse-field gel electrophoresis (PFGE) and automated repetitive extragenic palindromic sequence-based (rep-PCR) method (DiversiLab, Bacterial Barcodes, Inc.) for typing Finnish MRSA strains was studied. A total of 110 clinical MRSA isolates collected from well-defined outbreaks in Ostrobothnia region and 96 isolates from several epidemics or sporadic source...

Journal: :Journal of clinical microbiology 2013
Charlene R Jackson Johnnie A Davis John B Barrett

There is increasing interest in the presence of Staphylococcus aureus, specifically methicillin-resistant S. aureus (MRSA), on retail meat products. In this study, staphylococci were isolated from retail pork and retail beef in Georgia, and MRSA from the products was compared to human MRSA from the same geographic area using broth microdilution antimicrobial susceptibility testing, multilocus s...

2014
Nancy Younis Omar Hala Abdel Salam Ali Reem Abdel Hameed Harfoush Engy Hamdy El Khayat

Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) ...

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